Identification of leaf rust resistance gene Lr34/Yr18/Sr57/Pm38/Bdv1 in soft wheat varieties
Aim. Analysis of the allelic composition of the csLV34 locus and the microsatellite locus Xgwm295 for the detection of the Lr34/Yr18/Sr57/Pm38/Bdv1 gene, which determines the resistance of modern wheat varieties of domestic breeding of diseases: leaf rust and powdery mildew. Methods. Molecular genetic (DNA isolation, polymerase chain reaction, electrophoresis of amplification products) and phytopathological methods. Results. A study of soft wheat varieties of Ukrainian breeding was carried out. Two csLV34а and csLV34b alleles were identified that correlate with the absence and presence of the Lr34/Yr18/Sr57/Pm38/Bdv1 gene. 17.6 % of the cultivars tested were found to have the csLV34b allele, which is associated with the presence of the Lr34/Yr18/Sr57/Pm38/Bdv1 gene and resistance to brown rust. Conclusions. PCR revealed six varieties of soft wheat that contain the allele csLV34b, which is associated with the presence of the gene Lr34/Yr18/Sr57/Pm38/Bdv1. The information obtained can be used in breeding programs to create new varieties resistant to disease.
Keywords: leaf rust, powdery mildew, soft wheat, polymerase chain reaction, varieties, stability.
Singh R. Breeding durable adult plant resistance to stem rust in spring wheat: Progress made in a decade since the launch of the Borlaug Global Rust Initiative. BGRI Technical Workshop. Sydney, 2015.
Lagudah E.S., McFadden H., Singh R.P. Molecular genetic characterization of the Lr34/Yr18 slow rusting resistance gene region in wheat. Theor.Appl.Genet. 2006. Vol. 114. P. 21–30. doi: 10.1007/s00122-006-0406-z
Nenaidenko G.N., Mitin I.A. Fertilizer, fertility, productivity: Problems and prospects of development of agricultural science and agriculture in modern conditions. Ivanovo, 2003. P. 6–20. [in Russian].
Novohatka V.G. Creation of the source material for the selection of winter wheat resistant to powdery mildew. Sat scien-tific Proceedings of Mironov. Institute of Wheat. Mironovka, 1983. P. 116–126. [in Russian]
Krivchenko V.I. Study of the resistance of cereal crops to powdery mildew: method. directions. Leningrad, 1980. P. 78. [in Russian]
Peresipkin V.F. Atlas of twigs of Polish cultures. Kiev: Harvest, 1976. P. 102. [in Russian]
Lagudah E.S., McFadden H., Singh R.P. et al. Molecular genetic characterization of the Lr34/Yr18 slow rusting resistance gene region in wheat. Theor. Appl. Genet. 2006. 114. P. 21–30. doi: 10.1007/s00122-006-0406-z
Singh D., Park R.F., McIntosh R.A. Characterisation of wheat leaf rust resistance gene Lr34 in Australian wheats using components of partial resistance and molecular markers. Aust. J. Agric. Res. 2007. 58. P. 1106–1114. doi: 10.1071/AR07002
Kolmer J.A., Singh R.P., Garvin D.F. et al. Analysis of the Lr34/Yr18 rust resistance region in wheat germplasm. Crop Sci. 2008. 48. P. 1841–1852. doi: 10.2135/cropsci2007.08.0474
McCallum B.D., Somers D.J., Humphreys D.G., Cloutier S. Molecular marker analysis of Lr34 in Canada Western Red Spring wheat cultivars. Proc. 11th Int. genet. symp. Brisbane, 2008. P. 137–140.
Babayants L.T., Meshterkhazi A., Wechter F. Methods of selection and assessment of the resistance of wheat and barley to diseases in the CMEA member countries. Praga, 1988. P. 321. [in Russian]
Stewart C.N., Via L.E. A rapid CTAB DNA isolation technique useful for RAPD fingerprinting and other PCR applications. Bio. Techniques. 1993. 14 (5). P. 748–749.
Lagudah E.S., Krattinger S.G., Herrera-Foessel S. et al. Gene-specific markers for the wheat gene Lr34/Yr18/ Pm38 which confers resistance to multiple fungal pathogens. Theor. Appl. Genetics. 2009. Vol. 119. P. 889–898. doi: 10.1007/s00122-009-1097-z
Dyck P.L. Genetics of leaf rust reaction in three introductions of common wheat. Can. J. Genet. Cytol. 1977. Vol. 19. P. 711–716. doi: 10.1139/g77-077
Spielmeyer W, Singh R.P., McFadden H. et al. Fine scale genetic and physical mapping using interstitial deletion mutants of Lr34/ Yr18: a disease resistance locus eVective against multiple pathogens in wheat. Theor. Appl. Genetics. 2008. Vol. 116. P. 481–490. doi: 10.1007/s00122-007-0684-0
McIntosh R.A. Close genetic linkage of genes conferring adult-plant resistance to leaf rust and stripe rust in wheat. Plant Pathol. 1992. Vol. 41. P. 523–527. doi: 10.1111/j.1365-3059.1992.tb02450.x
Singh R.P. Genetic association of leaf rust resistance gene Lr34 with adult plant resistance to stripe rust in bread wheat. Phytopathology. 1992. Vol. 82. P. 835–838. doi: 10.1094/Phyto-82-835
Krattinger S.G., Lagudah E.S., Spielmeyer W. et al. A putative ABC transporter confers durable resistance to multiple fungal pathogens in wheat. Science. 2009. Vol. 323. P. 1360–1363. doi: 10.1126/science.1166453
Schnurbusch T., Paillard S., Schori A. et al. Dissection of quantitative and durable leaf rust resistance in Swiss winter wheat revealsa major resistance QTL in the Lr34 chromosomal region. Theor. Appl. Genetics. 2004. Vol. 108. P. 477–484. doi: 10.1007/s00122-003-1444-4
Suenaga K., Singh R.P., Huerta-Espino J., William H.M. Microsatellite markers for genes Lr34/Yr18 and other quantita-tive trait loci for leaf rust and stripe rust resistance in bread wheat. Phytopathology. 2003. Vol. 93. P. 881–890. doi: 10.1094/PHYTO.2003.93.7.881
Roder M.S., Korzun V., Wendehake K. et al. A microsatellite map of wheat. Genetics. 1998. Vol. 149. P. 2007–2023.
Radchenko O.M., Tishchenko O.M. Determination of the Lr 34 leaf rust resistance gene in common wheat varieties using a microsatellite marker. The Bulletin of Ukrainian Society of Geneticists and Breeders. Kiev, 2010. P. 41–45. [in Russian]
Galaev O.V., Syvolap Y.V. Characterization of wheat varieties of soft Ukrainian and Russian breeding by alleles of the csLV34 locus, linked to the multipathogenic resistance gene Lr34/ Yr18/Pm38. Cytology and genetics. 2015. Vol. 49, No. 1. P. 18–25. [in Ukrainian] doi: 10.3103/S0095452715010041