Genetic analysis of the content of palmytic acid glycerides in the carriers of maize mutation shrunken-1

Keywords: Zea mays L., sh1 mutation, palmitate content, genetic analysis


Aim. The influence of corn shrunken-1 (sh1) mutation on the content of palmitate in corn oil establishing and genetic analysis of this trait. Methods. The effects of the sh1 mutation on the content of palmitate were determined by comparing the inbreds – carriers of this mutation with inbreds of the common type, as well as in the top-crosses of common type inbreds with the mutant inbreds. The genetic components of the variance in terms of the content of palmitate were analyzed in the diallel crosses of mutant inbreds according to the second Griffing method. The determination of oil fatty acid composition was carried out by the Peisker gas chromatographic method. Results. It has been established that the corn inbreds – carriers of sh1 mutation exceed the common type inbreds by an average of 29.2 % in terms of palmitate content. The level of the trait in the inbreds based on the sh1 mutation was notable as having the quantitative variability and varied within 12.2-15.6 %. The content of palmitate in the carriers of sh1 mutation was inherited as incomplete dominance with a predominant contribution of additive effects to the variance. A higher level of the trait was controlled by recessive alleles of polygenes. Conclusions. The obtained results suggests the possibility of spatial linkage of the sh1 mutant gene with palmitate-coding locus, the effect of which is modified by the polygenic complex. It has been confirmed, that the carriers of sh1 mutation expands the genetic diversity of corn in terms of palmitate content.


Wang T., White P. Lipids of the kernel. Corn chemistry and technology : monograph/ S.O.Serna – Saldivar Ed., Cambrige – Duxford – Oxford : Elsevier Publ. – Woodhead Publ. 3rd Ed. 2019. Chpt. 13. P. 337–368. doi: 10.1016/B978-0-12-811971-6-00013-9.

Soukup J., Kourimska L. The effect of fatty acid profile on the stability of non- traditional and traditional plant oils. Slovak J. Food Sci. 2019. Vol. 13 (1). P. 744–750. doi: 10.5219/1064.

Ramana K. V., Srivastava S., Singhal S. S. Lipid peroxidation products in human health and disease. Oxidative Medicine and Cellular Longivity. 2019. Vol. 2019. Article ID 714235. doi: 10.1155/2019/71472235.

Grootveld M., Percival B. C., Leenders J., Wilson P. B. Potential adverse public health effects afforded by the ingestion of dietary lipid oxidation product toxins : significance of fried food sources. Nutrients. 2020. Vol. 12. Article 974. doi: 10.3390/nu12040974.

Murphy D. J. Using modern plant breeding to improve the nutritional and technological qualities of oil crops. Oilseeds & Fats Crops and Lipids. 2014. Vol. 21 (6). Article D607. doi: 10.1051/ocl/2014038.

Duvick S. A., Pollak L. M., Edwards J. W., White P. J. Altering the fatty acid composition of corn belt corn through Tripsacum introgression. Maydica. 2006. Vol. 51 (2). P. 409–416.

List G. R. Minor high – oleic oils. High oleic oils : development, properties and uses : monograph : F. Flider Ed. Cambrige – London – Oxford – San – Diego : AOCS Press – Acad. Press, 2021. Chpt. 6. P. 125–142. doi: 10.1016/B978-0-12-822912-5.00010-1.

White P., Pollak L. M., Duvick S. Improving the fatty acid composition of corn oil by using germplasm introgression. Lipid Technol. 2007. Vol. 19 (2). P. 35–38. doi: 10.1002/lite.200600009.

Yang X., Guo Y., Yan J., Zhang J., Song T., Rocheford T., Li J.-S. Major and minor QTL and epistasis contribute to fatty acid compositions and oil concentration in high-oil maize. Theor. Appl. Genet. 2010. Vol. 120 (3). P. 665–678. doi: 10.1007/s00122-009-1184-1.

Zhang K., Guo L., Cheng W., Liu B., Li W., Wang F., Xu C., Zhao X., Ding Z., Zhang K., Li K. Sh1-dependent maize seed development and starch synthesis via modulating carbohydrate flow and osmotic potential balance. BMC Plant Biology. 2020. Vol. 20 (1). Article 264. doi: 10.1186/s12870-020-02478-1.

Katyal V., Gangwar B. Statistical methods for agricultural field experiments. New Dehli : New India Publ. Agency, 2011. 160 p.

Tymchuk D. S., Sadovnichenko I., Tymchuk N., Potapenko H., Torianyk I. Oleic acid glycerides content in the oils of maize endospermic mutants and its dependence on temperature during ripening. Proc. Latvian Acad. Sci. Section B. 2021. Vol. 75(5). Р. 403–410. doi: 10.2478/prolas-2021-0059.

Hrytsyuk P. M., Ostapchuk O. P. Data analysis: study guide. Rivne : NUVMNM, 2008. 218 p. [in Ukrainian]

Litun P. P., Proskurnin N. V. Genetics of quantitative traits. Genetic crossings and genetic analysis : study guide. Kyiv : UMVO, 1992. 96 p. [in Russian]

Orhun G. E. Genetic control of oil and saturated fatty acids in maize (Zea mays L.) populations. J. Agr. Fac. Gas. Univ. 2018. Vol. 35 (3). P. 242–247. doi: 10.13002/jafag4388.