Properties of actinomycetes from the rhizosphere of Colobanthus quitensis (Kunth) Bartl. (Booth Island, maritime Antarctica)

Keywords: Antarctic bacteria, antimicrobial activity, enzyme production


Aim. Investigate the biological properties of actinomycetes from the rhizosphere of Colobanthus quitensis (Kunth) Bartl. and study their ability to produce bioactive compounds. Methods. Microbiological (isolation, synthesis of bioactive compounds), genetic and genetic engineering (isolation and analysis of total DNA, DNA gel electrophoresis, polymerase chain reaction, DNA sequencing), bioinformatic (phylogenetic analysis) methods. Results. 21 actinomycete isolates were obtained from the samples of C. quitensis rhizosphere. Half of the isolates exhibited antagonistic properties towards at least one of 17 test cultures of pathogenic and phytopathogenic microorganisms. Some strains exhibited both antibacterial and antifungal activities. The majority of isolates (70-95 %) produced proteases, amylases, cellulases, almost 42 % – nitrate reductases, 20-25 % – pectinases and lipases, respectively. Conclusions. A collection of Antarctic actinomycetes has been created and characterized, which is promising for evaluating their metabolic potential as producers of antibiotics.


Baskaran A., Kaari M., Venugopal G., Manikkam R., Joseph J., Bhaskar P. V. Anti freeze proteins (Afp): Properties, sources and applications – A review. International Journal of Biological Macromolecules. 2021. Vol. 189. P. 292–305. doi: 10.1016/j.ijbiomac.2021.08.105.

Edgar R. C. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic acids research. 2004. 32 (5). P. 1792–1797. doi: 10.1093/nar/gkh340

Gavriilidou A., Kautsar S. A., Zaburannyi N., Krug D., Müller R., Medema M. H., Ziemert N. Compendium of specialized metabolite biosynthetic diversity encoded in bacterial genomes. Nature microbiology. 2022. 7 (5). P. 726–735. doi: 10.1038/s41564-022-01110-2

Gromyko O., Tistechok S., Roman I., Aravitska O., Luzhetskyy A., Parnikoza I., Fedorenko V. Isolation and characterization of culturable actinobacteria associated with Polytrichum strictum (Galindez Island, the maritime Antarctic). Ukrainian Antarctic Journal. 2021. Vol. (1). P. 82–97. doi: 10.33275/1727-7485.1.2021.668.

Hui M. L. Y., Tan L. T. H., Letchumanan V., He Y. W., Fang C. M., Chan K. G., Lee L. H. The extremophilic actinobacteria: From microbes to medicine. Antibiotics. 2021. Vol. 10 (6). P. 682. doi: 10.3390/antibiotics10060682.

Jurelevicius D., Pereira R. D. S., da Mota F. F., Cury J. C., de Oliveira I. C., Rosado A. S., Seldin L. Metagenomic analysis of microbial communities across a transect from low to highly hydrocarbon‐contaminated soils in King George Island, Maritime Antarctica. Geobiology. 2022. Vol. 20 (1). P. 98–111. doi: 10.1111/gbi.12472.

Núñez-Montero K., Barrientos L. Advances in Antarctic research for antimicrobial discovery: a comprehensive narrative review of bacteria from Antarctic environments as potential sources of novel antibiotic compounds against human pathogens and micro-organisms of industrial importance. Antibiotics. 2018. Vol. 7 (4). P. 90. doi: 10.3390/antibiotics7040090.

Salwan R., Sharma V. The role of actinobacteria in the production of industrial enzymes. In New and future developments in microbial biotechnology and bioengineering. Elsevier, 2018. P. 165–177. doi: 10.1016/B978-0-444-63994-3.00011-4

Silva T. R., Duarte A. W., Passarini M. R., Ruiz A. L. T., Franco C. H., Moraes C. B., ... Oliveira V. M. Bacteria from Antarctic environments: diversity and detection of antimicrobial, antiproliferative, and antiparasitic activities. Polar Biology. 2018. Vol. 41. P. 1505–1519. doi: 10.1007/s00300-018-2300-y.

Tamura K., Stecher G., Kumar S. MEGA 11: molecular evolutionary genetics analysis version 11. Molecular biology and evolution. 2021. 38 (7). P. 3022–3027. doi: 10.1093/molbev/msab120

Tistechok S., Roman I., Fedorenko V., Luzhetskyy A., Gromyko O. Diversity and bioactive potential of Actinomycetia from the rhizosphere soil of Juniperus excelsa. Folia Microbiologica. 2023. P. 1–9. doi: 10.1007/s12223-023-01047-x