The influence of bacterization on the formation of the crop structure of soybean isogenic lines by E-genes in conditions of different photoperiod

Keywords: Glycine max (L.) Merr, E-genes, photoperiodic response, crop structure, bacterization, Bradyrhizobium japonicum

Abstract

Aim. To study the effect of treatment with the Bradyrhizobium japonicum 634b strain on the formation of the crop structure of soybean lines isogenic by photoperiodic sensitivity control genes, under conditions of long and short days. Methods. The experiment was done in the field. After sterilization, the seeds were treated with Bradyrhizobium japonicum 634b and planted in the soil; control test - seeds treated with distilled water. Plants were grown under natural long day conditions (16 h), in phase V3 the experimental plants were exposed to (artificially created) short day (9 h) for two weeks. The elements of the crop structure were analyzed. Results. In the short day condition and bacterization, we observe a significant decrease in the shoot length, the number of pods and seeds in short-day lines, and at the same time a significant increase in the indicators in long-day lines. In the long day condition, we observe more complex regularities, which, we assume, are associated with a closer and more specific interaction between the plant genotype and microorganisms. Conclusions. It was revealed that the genotype and its interaction with the bacterization factor has the greatest influence on the studied indicators, both under short and long day conditions.

References

The 2022 sowing campaign has been completed in Ukraine. Ministry of Agrarian Policy and Food of Ukraine. Retrieved from: https://minagro.gov.ua/news/v-ukrayini-zavershena-posivna-kampaniya-2022. [in Ukrainian]

Tsubokura Y., Watanabe S., Xia Z. et al. Natural variation in the genes responsible for maturity loci E1, E2, E3 and E4 in soybean. Ann. Bot. Vol. 113 (3). P. 429–441. doi: 10.1093/aob/mct269.

Xia Z., Watanabe S., Yamada T. et al. Positional cloning and characterization reveal the molecular basis for soybean maturity locus E1 that regulates photoperiodic flowering. Proceedings of the National Academy of Sciences. Vol. 109, No. 32. doi: 10.1073/pnas.1117982109.

Zhao C., Takeshima R., Zhu J. et al. A recessive allele for delayed flowering at the soybean maturity locus E9 is a leaky allele of FT2a, a FLOWERING LOCUS T ortholog. BMC Plant Biol. Vol. 16 (1). P. 20. doi: 10.1186/s12870-016-0704-9.

Mishra P., Panigrahi K. C. GIGANTEA – an emerging story. Frontiers in Plant Science. Vol. 6. 26.01.2015. doi: 10.3389/fpls.2015.00008.

Hayat R., Ahmed I., Sheirdil R. A. An Overview of Plant Growth Promoting Rhizobacteria (PGPR) for Sustainable Agriculture. Crop Production for Agricultural Improvement. ed. M. Ashraf., M. Öztürk., M. S. A. Ahmad. et al. Dordrecht : Springer Netherlands, 2012. P. 557–579. doi: 10.1007/978-94-007-4116-4_22.

Yang Y., Zhao Q., Li X. et al. Characterization of Genetic Basis on Synergistic Interactions between Root Architecture and Bio-logical Nitrogen Fixation in Soybean. Front. Plant Sci. Vol. 8. 23.08.2017. P. 1466. doi:10.3389/fpls.2017.01466

Integrating Genetics and Genomics to Advance Soybean Research. Retrieved from: https://www.soybase.org.

Melnykova N. M., Kots S. Ya. Effect of goat’s-rue Rhizobia on the formation and functioning of the soybean – Bradyrhizobium japonicum 634b symbiosis. Agriciltural microbiology. Vol. 29, 17.10.2019. P. 29–36. doi: 10.35868/1997-3004.29.29-36 [in Ukrainian].