Identification of the Glu-B1al allele encoding the overexpressed high-molecular-weight glutenin subunit Bx7oe in wheat lines using KASP technology

  • M. V. Halaieva Plant Breeding and Genetic Institute – National Center of Seed and Cultivar Investigation, National Academy of Agrarian Sciences of Ukraine, Ukraine, 65036, Odesa, Ovidiopolska Doroha, 3 https://orcid.org/0000-0001-8133-5184
  • O. V. Halaiev Plant Breeding and Genetic Institute – National Center of Seed and Cultivar Investigation, National Academy of Agrarian Sciences of Ukraine, Ukraine, 65036, Odesa, Ovidiopolska Doroha, 3 https://orcid.org/0000-0001-7247-2910
  • O. O. Pogrebniuk Plant Breeding and Genetic Institute – National Center of Seed and Cultivar Investigation, National Academy of Agrarian Sciences of Ukraine, Ukraine, 65036, Odesa, Ovidiopolska Doroha, 3 https://orcid.org/0009-0004-3256-297X
  • V. I. Fait Plant Breeding and Genetic Institute – National Center of Seed and Cultivar Investigation, National Academy of Agrarian Sciences of Ukraine, Ukraine, 65036, Odesa, Ovidiopolska Doroha, 3 https://orcid.org/0000-0001-9994-341X
  • M. Rahmatov Swedish University of Agricultural Sciences, Sweden, SE-23053, Alnarp https://orcid.org/0000-0001-7491-2836
DOI: https://doi.org/10.7124/FEEO.v36.1713
Keywords: common wheat (Triticum aestivum L.), recombinant inbred lines, KASP markers, high-molecular-weight glutenin, gene, allele

Abstract

Aim. The aim of the study was to characterize F7 recombinant inbred lines (RILs) derived from the cross Luzanivka odeska / Odeska chervonokolosa and their parental forms with respect to the Glu-B1al allele using KASP technology, as well as to assess the impact of this allele on grain yield and its components. Methods. Genotyping was performed using competitive allele-specific PCR (KASP) with fluorescence detection. Phenological observations, morphometric traits, and yield structure elements were evaluated. Statistical analysis was conducted using Microsoft Excel, and the significance of differences among variants was assessed using Fisher’s F-test. Results. The parental varieties and the F F7 RIL population were analyzed for the Glu-B1 gene. It was found that the variety Odeska chervonokolosa and 53 lines carried the Glu-B1al allele, which encodes the overexpressed Bx7OE high-molecular-weight glutenin subunit associated with improved bread-making quality. The variety Luzanivka odeska and 45 lines lacked this allele, and one line was heterozygous. The KASP genotyping results were compared with field evaluation data for eight agronomic traits: heading time, plant height, productive tillering, number and weight of grains per spike, 1000-kernel weight, number of productive stems, and grain yield. Conclusions. The presence of the Glu-B1al allele, associated with improved flour quality, had no negative impact on key agronomic traits. Selection for this allele may be an effective tool in wheat breeding programs aimed at improving quality without compromising yield, even under stress conditions.

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