Ivermectin’s influence on morpho-physiological parameters of Arabidopsis thaliana L. roots

  • Y. O. Kustovskiy Institute of Food Biotechnology and Genomics, Natl. Acad. Sci. Ukraine, Ukraine, 04123, Kyiv, Baidy-Vyshnevetskoho str., 2А; National University of Kyiv Mohyla Academy, Ukraine, 04070, Kyiv, Skovorody str., 2 https://orcid.org/0000-0002-1536-3897
  • A. I. Yemets Institute of Food Biotechnology and Genomics, Natl. Acad. Sci. Ukraine, Ukraine, 04123, Kyiv, Baidy-Vyshnevetskoho str., 2А; National University of Kyiv Mohyla Academy, Ukraine, 04070, Kyiv, Skovorody str., 2 https://orcid.org/0000-0001-6887-0705
DOI: https://doi.org/10.7124/FEEO.v37.1764
Keywords: ivermectin, Arabidopsis thaliana L., root, morphological properties

Abstract

Aim. To study ivermectin’s influence on morphology of roots and root hairs of Arabidopsis thaliana L. seedlings. Methods. In vitro seedlings’ cultivation, microscopy, statistical data analysis. Results. Morphological changes of roots were determined after 12 days of seedlings growth on the nutrient medium (Murashige and Skoog) with ivermectin in concentration of 250 µg/ml. It was determined that ivermectin at the concentration of 250 µg/ml causes main roots curling, elongation zone shortening, lateral roots growth retardation, decrease in length and deformation of root hairs. Statistically significant differences in length of root hairs were observed on the 12th day of cultivation. Particularly, the mean length of root hairs was 163.63±57.97 µm in seedlings, which grew on medium without ivermectin, and 28.14±9.36 µm in seedlings, which grew on medium with 250 µg/ml of ivermectin. Conclusions. Morphological alterations of roots and root hairs сaused by 250 µg/ml of ivermectin were analyzed. According to the proposed explanation of the obtained results, ivermectin disrupts arrangements of microtubules in cells of A. thaliana main root leading to pathological changes in roots’ morphology.

References

Martin R. J., Robertson A. P., Choudhary S. Ivermectin: an anthelmintic, an insecticide, and much more. Trends. Parasitol. 2021. Vol. 37 (1). P. 48–64. https://doi.org/10.1016/j.pt.2020.10.005.

Syslová E., Landa P., Navrátilová M. et al. Ivermectin biotransformation and impact on transcriptome in Arabidopsis thaliana. Chemosphere. 2019. Vol. 234. P. 528–535. https://doi.org/10.1016/j.chemosphere.2019.06.102.

Vokřál I., Michaela Š., Radka P. et al. Ivermectin environmental impact: Excretion profile in sheep and phytotoxic effect in Sinapis alba. Ecotoxicol. Environ. Saf. 2019. Vol. 169. P. 944–949. https://doi.org/10.1016/j.ecoenv.2018.11.097.

Navrátilová M., Raisová Stuchlíková L., Moťková K. et al. The uptake of ivermectin and its effects in roots, leaves and seeds of soybean (Glycine max). Molecules. 2020. Vol. 25 (16). P. 1–10. https://doi.org/10.3390/molecules25163655.

Navrátilová M., Raisová Stuchlíková L., Skálová L. et al. Pharmaceuticals in environ-ment: the effect of ivermectin on ribwort plantain (Plantago lanceolata L.). Environ. Sci. Pollut. Res. Int. 2020. Vol. 27 (25). P. 31202–31210. https://doi.org/10.1007/s11356-020-09442-4.

Langhansová L., Navrátilová M., Skálová L., Mot’ková K., Podlipná R. The effect of the manure from sheep treated with anthel-mintics on clover (Trifolium pratense). Agronomy. 2021. Vol. 11 (9). P. 1–14. https://doi.org/10.3390/agronomy11091892.

Laber L., Eichberg C., Zimmerbeutel A., Düring R. A., Donath T. W. Effects of macrocyclic lactone anthelmintics on seed germination of temperate grassland species. Plant Biol. (Stuttg). 2023. Vol. 25 (7). P. 1046–1057. https://doi.org/10.1111/plb.13577.

Kustovskiy Y. O., Karpov P. A., Blume Y. B., Yemets A.I. Ivermectin affects Arabidopsis thaliana microtubules through predicted binding site of β-tubulin. Plant Physiol. Biochem. 2024. Vol. 206. 108296. P. 1–12. https://doi.org/10.1016/j.plaphy.2023.108296.

Schindelin J., Arganda-Carreras I., Frise E. et al. Fiji: an open-source platform for biological-image analysis. Nat. Methods. 2012. Vol. 9 (7). P. 676–682. https://doi.org/10.1038/nmeth.2019.

Hsiao A. S., Huang J. Y. Microtubule regulation in plants: from morphological development to stress adaptation. Biomolecules. 2023. Vol. 13 (4). P. 1–18. https://doi.org/10.3390/biom13040627.