Influence of iron source in induction medium on haplopreductive capacity of bread wheat in in vitro anther culture

  • O. L. Shestopal Plant Breeding and Genetic Institute – National Center of Seed and Cultivar Investigation, Ukraine, 65036, Odesa, Ovidiopolska Doroha, 3 https://orcid.org/0000-0002-2987-9712
  • I. S. Zambriborshch Plant Breeding and Genetic Institute – National Center of Seed and Cultivar Investigation, Ukraine, 65036, Odesa, Ovidiopolska Doroha, 3 https://orcid.org/0000-0003-2430-3690
  • O. A. Afinogenov Plant Breeding and Genetic Institute – National Center of Seed and Cultivar Investigation, Ukraine, 65036, Odesa, Ovidiopolska Doroha, 3 https://orcid.org/0009-0000-9392-0233
  • M. S. Chekalova Plant Breeding and Genetic Institute – National Center of Seed and Cultivar Investigation, National Academy of Agrarian Sciences of Ukraine, Ukraine, 65036, Odesa, Ovidiopolska Doroha, 3 https://orcid.org/0000-0001-7505-8459
  • O. A. Vasyliev Plant Breeding and Genetic Institute – National Center of Seed and Cultivar Investigation, National Academy of Agrarian Sciences of Ukraine, Ukraine, 65036, Odesa, Ovidiopolska Doroha, 3
  • V. A. Traskovetska Plant Breeding and Genetic Institute – National Center of Seed and Cultivar Investigation, National Academy of Agrarian Sciences of Ukraine, Ukraine, 65036, Odesa, Ovidiopolska Doroha, 3
  • N. I. Sauliak Plant Breeding and Genetic Institute – National Center of Seed and Cultivar Investigation, National Academy of Agrarian Sciences of Ukraine, Ukraine, 65036, Odesa, Ovidiopolska Doroha, 3
DOI: https://doi.org/10.7124/FEEO.v37.1771
Keywords: dihaploids, wheat, androgenesis, callus, regeneration

Abstract

Aim. Study of the efficiency of in vitro androgenesis of bread wheat using different sources of iron in the induction medium for anther cultivation. Methods. In vitro culture of isolated anthers. The following were determined: the percentage of callus formed and the percentage of green plants from planted anthers; the percentage of acclimatized plants from green regenerants; the percentage of fertile plants from acclimatized plants and from planted anthers. Results. Androgenesis in vitro was studied in anther culture of 11 genotypes of bread winter wheat. Callus induction frequency: from 0.65 to 18.46 %, on average 6.68 % – on a medium with ferylene and from 0.53 to 35.58 %, on average 10.85 % – on a medium with Fe-chelate. The ability to regenerate green plants: from 0 to 4.03 %, on average 6.68 % – on a medium with ferylene and from 0 to 4.11 %, on average 10.85 % – on a medium with Fe-chelate. 35 dihaploid lines were obtained in anther culture of eight genotypes. Spontaneous diploidization on average 30.7 %. Conclusions. Genotype-specific morphogenetic reactions of bread winter wheat microspores during in vitro androgenesis have been revealed. It has been shown that the use of Fe-chelates (Na2EDTA + FeSO4•7H2O) as an iron source in the induction nutrient medium is more effective for obtaining dihaploids by in vitro androgenesis.

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