Genetic diversity of barley (Hordeum vulgare L.) in capability for haploid production in anther culture in vitro

DOI: https://doi.org/10.7124/FEEO.v38.1817
Keywords: Hordeum vulgare L., barley, anther culture in viro, morphogenic anthers, plant regeneration

Abstract

Aim. Analysis of data of the long-term investigations on the ability of spring barley varieties and lines differed in origin, biological and valuable agronomic traits for haploid production in anther culture in vitro. Methods. Anthers isolated from cold-pretreated spikes were cultivated on agar and starch solidified nutrient media containing N6 macro-and MS micronutrients, physiologically active substances and maltose (9.0%). Results. The capacities for production of calli, embryoids and plant regeneration were investigated in 30 varieties, three collection accessions and three doubled haploid lines. Response to androgenesis in vitro varied from 5.70 to 91.71% of morphogenic anthers and from 0 to 145.9 green plants per 100 cultivated anthers in dependence of genotype, donor plant growth conditions and culture medium. Eight genotypes that combined middle and high (20‒70 green regenerated plants per 100 anthers) capacities for haploid production with certain valuable agronomic characteristics were found. Conclusions. This work emphasizes the importance of further screening barley gene pool for selection of genotypes with a unique high cultuability as well as the improvement of special methods for increase haplod production in recaicitrant ones.

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