Genetic polymorphism of malate dehydrogenase and glutamate oxalacetate transaminase in coeval Pinus pallasiana D.Don trees with different stem diameter

  • А. Р. Багдасарова
  • И. И. Коршиков
  • Л. А. Калафат
  • С. Н. Привалихин
  • Н. Н. Пирко
  • А. С. Постовойтова
  • Я. В. Пирко

Abstract

Aims. Genetic characteristics of coeval Pinus pallasiana D. Don trees with different stem diameters were studied. Methods. Pine vegetative buds tissues were used for electrophoretic analysis of isoenzymes of malate dehydrogenase and glutamate oxalacetate transaminase. Results. The analysis of 220 pine trees was made. Mean observed heterozygosity, genetic heterogeneity and Ney distances among tree groups with different stem diameters were calculated. Coefficient of correlation between heterozygosity and stem diameter was also calculated. Conclusions. Observed heterozygosity among trees with different stem diameters ranges insignificantly. There is a tendency toward the increased heterozygosity by Mdh-3 locus in tree groups with greater stem diameter. Coefficients of correlation between heterozygosity and stem diameter by GOT and MDH loci are low.

Keywords: molecular markers, isoenzymes, Pinus pallasiana D. Don, genetic polymorphism.