Influence of plant growth regulators on the regeneration of shoots of plants Hypericum perforatum L. and Taraxacum officinalis L.

Abstract

Aims. The aim of our work was to compare the frequency of plant regeneration of H. perforatum from the leaves, internodes and roots and frequency of plant regeneration Т. officinalis using the proximal, middle and distal part of a leaf. We also determined the optimal type of an explant for maximal plant regeneration frequency. Methods. For induction of regeneration of H. perforatum and Т. officinalis shoots we used MS culture medium added with BAP, kinetin and NAA. We used leaves, internodes and roots of the H. perforatum aseptic plants. We also used proximal, middle and distal parts of Т. officinalis leaves. Results. MS medial supplemented by NAA and different cytokinins differed in their ability to induce plant regeneration. The presence of these growth regulators almost in all cases stimulated the formation of shoots for H. perforatum explants. Using of kinetin in MS medium have led to inhibition of plant regeneration from H. perforatum leaves and resulted in low (20–60 %) regeneration frequency of Т. officinalis. Using of BAP and NAA was the most effective for increasing of plant regeneration frequency of H. perforatum and Т. officinalis up to 100 %. Conclusions. H. perforatum internodes and proximal parts of T. officinalis leaves possessed the greatest regenerative ability. The proximal, medial and distal leaves parts have differed in sensitivity to growth regulators. The maximum regeneration rate of 100 % was observed cultivating proximal leaves on three different media of 12 investigated.

Keywords: Hypericum perforatum, Taraxacum officinalis, regeneration, 6-benzylaminopuryn, kinetin.