Obteining of transgenic Nicotiana benthamiana plants carrying genes of prolyl-4-hydroxylase subunits

Abstract

Aims. The use of plants as a recombinant peptides expression system is a valuable alternative for the production of collagen for various medical and scientific purposes. The aim of this work was to obtained transgenic plants of Nicotiana benthamiana with two human genes encoding subunits of prolyl-4-hydroxylase (P4H), responsible for key posttranslational modifications of collagen. Methods. Transgenic plants were obtained via Agrobacterium mediated transformation method. Presence of the target and selective genes was confirmed by PCR-analysis. Results. We report here the development of transgenic plants of Nicotiana benthamiana carrying genes of prolyl-4-hydroxylase (P4H) subunits. Conclusions. Obtained plants have potential to produce adequately modified exogenous collagen with mammalian-like post-translation modifications via transient expression experiments.

Keywords: Nicotiana benthamiana, transgenic plants, prolyl-4-hydroxylase, collagen, transient expression.