Identification of modern and ancient Beijing strain sublineages in Kyiv’s population of Mycobacterium tuberculosis

Abstract

Aim. Characterization of M. tuberculosis Beijing family in Kyiv’s clinical strains population by content of «modern» and «ancient» sublineages as defined by structure of NTF locus. Methods. Multiplex PCR of NTF locus, ETR-VNTR, and SNP-typing (katG⁴⁶³) of bacterial isolate DNA samples from Kyiv’s patients with pulmonary tuberculosis. Results. 19 clinical isolates of M. tuberculosis strains belonged to the PGG-1 by SNP-typing and to two of the most prevalent ETR-VNTR Beijing genotypes (42435, 42434) showed NTF-locus structure characteristic to «modern» type. Conclusions. More samples should be analyzed to determine the proportion of «ancient» Beijing subtypes in Kyiv M. tuberculosis population. Alternative quick and convenient methods for detection of these sublineages could be considered for more effective strategy of M. tuberculosis monitoring in Kyiv.

Keywords: Mycobacterium tuberculosis, molecular epidemiology, NTF-locus.