Regenerative ability of sunflower cultivated lines (Helianthus annuus L.) in vitro

  • В. О. Бабич
  • С. В. Хомутовська
  • О. Ю. Куліш
  • В. А. Смірнова
  • Я. Ф. Парій
  • М. Ф. Парій
  • Ю. В. Симоненко


Aim. Sunflower (Helianthus annuus L.) is one of the most important oil crops in the word. The application of biotechnological methods for the study and the improvement of the sunflower is mainly limited by the difficulty of regeneration of plants in a reproducible and efficient fashion. Therefore an efficient plant regeneration system is necessary for successful use and improvement of cultural sunflower. Methods. Cultivation of different types of sunflower explants in vitro. Results. The study of regeneration capacity of sunflower shows the difference between the ratio and concentration of phytohormones and type ofexplants that were used in our experiments. The regeneration frequency of sunflower in vitro varies depending on the type of explants, genotype and composition of the nutrient medium. Conclusions. In the study of different genotypes of sunflower we have shownthat hypocotyls are better as explants than cotyledons and roots due to their regeneration capacity. Regeneration efficiency for this type of explants was 6–14%.

Keywords: Sunflower (Helianthus annuus L.), plant regeneration, hypocotyls, cotyledons.