Effect of growth regulators on realization of spring barley (Hordeum vulgare L.) morphogenic potential in anther culture in vitro

Abstract

Aim. Growth regulators (natural and synthetic phytohormones) are known to be an important factor for morphogenesis in plant cell, tissue and organ culture in vitro. It has been shown that genotypic differences for androgenetic ability could be explained by discrepancy between endo- and exogenous phytohormone contents. From this point of view, genotypic dependence would be overcome via adjustment of media hormone composition. Investigations aimed to elucidate responses of genotypes with different in vitro androgenic ability to auxin- and cytokinin-active growth regulator contents in inductive medium.Methods. Plants were grown under field conditions. Anthers were isolated from spikes of two spring barley cultivars and DH-line and inoculated on inductive media contained N6 macro-, MS micronutrients, organic supplements and different concentrations of 2.4-D and BAP. Results. Genotypic dependence of response to plant growth regulator composition in nutrient medium for anther culture in vitro was confirmed. It was ascertained that genotype with high androgenic capacity did not require auxin for androgenic structure induction unlike genotype with low expression of this trait.The best yield of androgenic structures and plants was obtained on the filter sterilized media with combination of 2.4-D 2.0 mg/L and BAP 0.5 mg/L. Conclusions. Regardless of auxin- and cytokinin-active growth regulator contents and their ratios, androgenic capacity ranks of genotypes remained unchanged. As methodological approach aimed to overcome genotypic dependence cultivation of anthers isolated from spikes of genotypes with contrast androgenetic ability on media containing different growth regulator content has been appeared ineffective.

Keywords: HordeumvulgareL., spring barley, anther culture in vitro, growth regulators, morphogenesis.