Competence for regeneration on bulblet explants of rare and endangered plant of Lilium martagon L.

  • E. E. Kostyukova
  • I. Ya. Nam
  • V. V. Zayakin

Abstract

Purpose. In this study organogenic capacity of  bulblet of Lilium martagon L. was examined. Methods. The effect of different plant growth regulators on regeneration of L. martagon was studied on Murashige and Skoog’s (MS) medium. For regeneration different plant growth regulators added to MS basal medium were used. Results. Our results indicate that indole acetic acid and 6- benzilaminopurine promoted shoot regeneration and root formation from bulblet explants. Plantlets were acclimatized well in a greenhouse conditions. Conclusions. Best results was obtained on MS basal medium with 0.25mg/l 6- benzilaminopurine and  0.5mg/l indole acetic acid.

Key words: Lilium martagon L., plant growth regulators, regeneration, bulblet explants.