The lifespan of starving flies of different Drosophila melanogaster stocks: effects of caffeine and He-Ne laser light

  • O. V. Gorenskaya
  • N. S. Filiponenko
  • Yu. G. Shckorbatov


Aim. Analysis of the chronic effects of caffeine and laser radiation on starvation resistance in Drosophila melanogaster, in depends on the genotype. Methods. The experiments were carried out in wild type stocks of Drosophila melanogaster: Canton-S and Oregon; mutant stock ebony and stocks with mutation ebony saturated by stocks Canton-S and OregonebonyC-S and ebonyOr. Caffeine was applied in concentration of 0.5 mg/ml in the cultural medium. Flies were exposed to He-Ne laser light of wavelength 632.8 nm and surface power density 0.03 mW/cm2 for 5 minutes. Control flies were grown under standard living conditions. Results. The lifespan during starvation dependence on a genotype (17.62 % and 19.51 %), external factors (h = 17.02 % and 19.64 %) and on the combined factors – h = 4.37 % and 2.42 % (for males and males, respectively) was shown. Conclusions. Almost in all experimental variants caffeine and laser light induced the extension lifespan during starvation. The simultaneous applying of both factors (caffeine+laser light) induced the maximal lifespan extension in Drosophila stocks C-S, ebony, and ebonyOr. Such additive effect was not observed in ebonyC-S flies. We connect the observed effects of caffeine and laser light with hormesis effect.
Keywords: drosophila, genotype, lifespan during starvation, mutation, stress tolerance.


Lin Y.J., Seroude L., Benzer S. Extended life-span and stress resistance in the Drosophila mutant methuselah. Science. 1998. Vol. 282 (5390). P. 943–946.

Cooper T.M., Mockett R.J., Sohal B.H., Sohal R.S., Orr W.C. Effect of caloric restriction on life span of the housefly, Musca domestica. The FASEB journal. 2004. Vol. 18. P. 1591–1593. doi: 10.1096/fj.03-1464fje.

Calabrese E.J., Dhawan G., Kapoor R., Iavicoli I., Calabrese V. What is hormesis and its relevance to healthy aging and longevity? Biogerontology. 2015. Vol. 16 (6). P. 693–707. doi: 10.1007/s10522-015-9601-0.

Tiwari K.K., Chu C., Couroucli X., Moorthy B., Lingappan K. Differential concentration-specific effects of caffeine on cell viability, oxidative stress, and cell cycle in pulmonary oxygen toxicity in vitro. Biochemical and biophysical research communications. 2014. Vol. 450 (4). P. 1345–1350. doi: 10.1016/j.bbrc.2014.06.132.

Gorenskaya О.V. Formation of the adaptability of Drosophila melanogaster at the chronic action of caffeine The Journal of V.N. Karazin Kharkiv National University. Series: biology. 2010. Vol. 11 (905). P. 66–77.

Sassoli C., Chellini F., Squecco R., Tani A., Idrizaj E., Nosi D. Low intensity 635 nm diode laser irradiation inhibits fibroblast-myofibroblast transition reducing TRPC1 channel expression/activity: New perspectives for tissue fibrosis treatment. Lasers Surg Med. 2015. Vol. 48 (3). P. 318–332. doi: 10.1002/lsm.22441.

Tominaga R. Effects of He-Ne laser irradiation on fibroblasts derived from scar tissue of rat palatal mucosa. Kokubyo Gakkai Zasshi. 1990. Vol. 57 (4). P. 580–594.

Da Lage J-L., Capy P., David J-R. Starvation and desiccation tolerance in Drosophila melanogaster adults: Effects of environmental temperature. Journal of Insect Physiology. 1989. Vol. 35 (6). P. 453–457.

Marron M.T., Markow T.A., Kain K.J., Gibbs A.G. Effects of starvation and desiccation on energy metabolism in desert and mesic Drosophila. J. Insect Physiol. 2003. Vol. 49 (3). P. 261–270.

Harbison S.T., Chang S., Kamdar K.P., Mackay T.F. Quantitative genomics of starvation stress resistance in Drosophila. Genome Biol. 2005. Vol. 6 (4). R36.

Moskalev A., Zhikrivetskaya S., Krasnov G., Shaposhnikov M., Proshkina E., Borisoglebsky D. A comparison of the transcriptome of Drosophila melanogaster in response to entomopathogenic fungus, ionizing radiation, starvation and cold shock. BMC Genomics. 2015. Vol. 16 (13). S8. doi: 10.1186/1471-2164-16-S13-S8

Shaposhnikov M., Proshkina E., Shilova L., Zhavoronkov A., Moskalev A. Lifespan and stress resistance in drosophila with overexpressed DNA repair genes. Scientific Reports. 2015. Vol. 5. P. 15299. doi: 10.1038/srep15299

Kannan K., Fridell Y.W. Functional implications of Drosophila insulin-like peptides in metabolism, aging, and dietary restriction. Front Physiol. 2013. Vol. 16 (4). P. 288. doi: 10.3389/fphys.2013.00288.

Broughton S.J., Piper M.D.W., Ikeya T., Bass T.M., Jacobson J., Driege Y. Longer lifespan, altered metabolism, and stress resistance in Drosophila from ablation of cells making insulin-like ligands. PNAS. 2005. Vol. 102 (8). P. 3105–3110.

Karu T., Pyatibrat L. Gene expression under laser and light-emitting diodes radiation for modulation of cell adhesion: Possible applications for biotechnology. IUBMB Life. 2011. Vol. 63 (9). P. 747–753.

Harshman L. G., Haberer B.A. Oxidative stress resistance: a robust correlated response to selection in extended longevity lines of Drosophila melanogaster? J Gerontol A Biol Sci Med Sci. 2000. Vol. 55 (9). P. 415–417.

Tettweiler G., Miron M., Jenkins M., Sonenberg N., Lasko P. F. Starvation and oxidative stress resistance in Drosophila are mediated through the eIF4E-binding protein, d4E-BP. Genes Dev. 2005. Vol. 19 (16). P. 1840–1843.

Shi X., Dalal N.S., Jain A.C. Antioxidant behavior of caffeine: efficient scavenging of hydroxyl radicals. Food Chem Toxicol. 1991. Vol. 29 (1). P. 1–6.

Farivar S., Malekshahabi T., Shiari R. Biological effects of low level laser therapy. J Lasers Med Sci. 2014. Vol. 5 (2). P. 58–62.