Molecular-genetic testing of freshwater species and breeds of animals (fish, narrow-clawed crayfish, mollusks)

  • S. E. Dromashko
  • A. M. Slukvin
  • O. Yu. Koneva
  • A. A. Rouba
  • M. A. Sasinovich
  • S. N. Shevtsova


Aim. Description of the results of molecular genetic studies of a number of fish, narrow-clawed crayfish, and mollusk species. Methods. For the analysis, RAPD, SSR, and DNA-barcoding methods were used. Results. Unique RAPD amplicones were identified to differentiate Lahvinsky, Tremlyansky and Isobelinsky carp breeds. With the use of SSR markers, the Dniester-Dnieper and Kama population belonging to the sterlet, grown in two farms of Belarus, was established. An instruction on the application of molecular genetic analysis to establish the species belonging to meat and caviar of sturgeon species was developed. The species composition and genetic polymorphism in narrow-clawed crayfish in reservoirs of Brest and Gomel regions was estimated. The work on DNA-barcoding of mollusks has begun. Conclusions. Molecular-genetic passports of carp breeds of Belarusian breeding are compiled. The population belonging to sterlet is determined in two fish farms inBelarus. Instruction for the use of molecular genetic analysis to establish the species (population) belonging to the sturgeon fish and their products is introduced in the country and used for testing and certification of sturgeon products. For the Byelorussian Polesie region, it is established that the narrow-clawed crayfish in theGomel region reservoirs has the greatest genetic diversity.

Keywords: molecular markers, carp, sturgeon, narrow-clawed crayfish, mollusks.


NIH Genomic Data Sharing Policy, NOT-OD-14-124. National Institutes of Health. URL: (last accessed: 03.08.2017).

Nagoya Protocol on Access to Genetic Resources and the Fair and Equitable Sharing of Benefits Araising from their Utilization to the Convention ob Biological Diversity. World Intellectual Property Organization. URL: details.jsp?id=12294 (last accessed: 03.08.2017).

Dromashko S.E., Koneva O.Yu. Genetic certification of Belarusian carp breeds. The Science and Innovations. 2010. No. 11 (93). P. 36–40.

Dromashko S., Koneva O., Rouba E. Genotyping of Cyprinus carpio L. carps of Belarusian breeding. Saarbrücken, Germany: LAP LAMBERT Academic Publishing, 2017. 100 p.

Rouba E.A., Koneva O.Yu., Dromashko S.E. Estimation of genetic diversity for Belarusian carp breeds using microsatellite markers. Molecular and Applied Genetics: Proceedings. 2012. Vol. 13. P. 64–73.

Lesiuk M.I., Koneva O.Yu., Rouba E.A., Slukvin A.M. Molecular genetic studies of sterlet breeders (Acipenser ruthenus L.). Molecular and Applied Genetics: Proceedings. 2012. Vol. 13. P. 110–117.

Dromashko S.E., Slukvin A.M., Koneva O.Yu., Rouba E.A. Genetic analysis in determining the species belonging to the family of sturgeon fish and products from them. Kachestvo i ekologicheskaya bezopasnost pishchevykh produktov i proizvodstv: materialy Mezhdunarodnoy nauchnoy konferentsii s elementami nauchnoy shkoly dlya molodezhi. Tver: Tverskoy gosudarstvenny universitet, 2017. P. 10–14.

Sasinivich M.A., Slukvin A.M., Alekhnovich A.V. Use of bioinformatics methods in studying the genome in populations of decapod crayfish. Matematicheskiye metody v tekhnike i tekhnologiyakh: sbornik trudov mezhdunarodnoy nauchnoy konferentsii: in 12 vols. Vol. 12, Pt. 2. SPb.: Izdatelstvo Politekhnicheskogo universiteta, 2017. P. 70–75.

Dromashko S.E., Shevtsova S.N., Babenko A.S. Lead and cadmium influences on the metallothionein expression level in Lymnaea stagnalis L. adults. Factors in Experymental Evolution of Organisms. 2017. Vol. 21. P. 281–285.