Analysis of ukrainian and foreign wheat samples for the presence of stem rust resistance genes using molecular markers

  • B. V. Ivashchuk
  • Ya. V. Pirko
  • S. I. Spivak
  • A. I. Yemets
  • L. O. Kalafat
  • A. V. Karelov
  • N. O. Kozub
  • Ya. B. Blume


Aim. The aim of the study was a search of Sr2 and Sr33 gene resistance alleles in foreign and domestic wheat samples using molecular genetic markers. Methods. The PCR with provided molecular genetic markers and with specific primers of own design was used; CTAB method was used for DNA extraction; electrophoresis in agarose gel was used. Results. The analysis of 16 wheat samples using molecular genetic markers for stem rust resistance genes Sr2 and Sr33 was performed. In general, among 16 analyzed samples only two have Sr2 gene resistance allele, and another two – Sr33 gene resistance allele. There were no alleles of resistance of Sr-genes among the sorts of Ukrainian selection (Kharkivska 6 and Kharkivska 12), which were used in this study. Conclusions. There were four samples among the wheat lines/sorts with valuable traits of domestic and foreign selection that have Sr2 and Sr33 gene resistance alleles.

Keywords: wheat, molecular and genetic markers, resistance genes, stem rust.


McIntosh, R.A. Wheat Rusts: An Atlas of Resistance Genes. Canberra; Australia: CSIRO, 1995. 205 p.

Schumann G.L. Stem rust of wheat (black rust). The Plant Health Instructor. 2000. doi: 10.1094/PHI-I-2000-0721-01

Pretorius Z.A., Singh R.P., Payne T.S. Detection of virulence to wheat stem rust resistance gene Sr31 in Puccinia graminis f. sp. tritici in Uganda. Plant Disease. 2000. Vol 84(2). P. 203.

Stokstad E. Deadly wheat fungus threatens world's breadbaskets. Science. 2007. Vol. 315. P.1786–1787.

Loughman R. Yield loss and fungicide control of stem rust of wheat. Austral. J. Agricult. Res. 2005. Vol. 56. P. 91–96.

Doyle J.J., Doyle J.L. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bull. 1987. Vol. 19. P. 11–15.

Das B.K., Saini A., Bhagwat S.G., Jawali N. Development of SCAR markers for identification of stem rust resistance gene Sr31. Plant Breed. 2006. Vol. 125. P. 544–549.

Tomar S.M. Genes for resistance to rusts and powdery mildew in wheat. Indian Agricultural Research Institute: New Delhi, India, 2001. 152 p.

Singh R.P., Hodson D.P., Jin Y. Emergence and Spread of New Races of Wheat Stem Rust Fungus: Continued Threat to Food Security and Prospects of Genetic Control. Phytopathol. 2015. Vol. 105(7). P. 872–884.

Singh R.P., Hodson D.P., Huerta-Espino J. The emergence of Ug99 races of the stem rust fungus is a threat to world wheat production. Annu. Rev. Phytopathol. 2011. Vol. 49. P. 465–481.

Pretorius Z.A., Szabo L.J., Boshoff W.H.P. First Report of a New TTKSF Race of Wheat Stem Rust (Puccinia graminis f. sp. tritici) in South Africa and Zimbabwe. Plant Disease. 2012. Vol. 96(4). P. 590.

Fetch T., Zegeye T., Park R.F. Detection of Wheat Stem Rust Races TTHSK and PTKTK in the Ug99 Race Group in Kenya in 2014. Plant Disease. 2016. Vol. 100(7). P. 1495–1495.

Patpour M., Hovmøller S.M., Justesen A.F. Emergence of Virulence to SrTmp in the Ug99 Race Group of Wheat Stem Rust, Puccinia graminis f. sp. tritici, in Africa. Plant Disease. 2016. Vol. 100(2). P. 522–552.

Watson I.A. Long distance transport of spores of Puccinia graminis tritici in the southern hemisphere. Proceedings of the Linnean Society of New South Wales. 1983. Vol. 106. P. 311–321.

Ivaschuk B.V., Pirko YA.V., Blume Ya.B. Nucleotide-binding domein sequences of NB-LRR class protein usage for screening of stem rust resistance genes. Factors in experimental evolution of organisms.2016. Vol. 19. P. 25–32.

Ivaschuk B.V., Pirko Ya.V., Galkin A.P., Blume Ya.B. Sr33 and Sr35 gene homolog identification in genomes of cereals related to Aegilops tauschii and Triticum monococcum. Cytol.Genet. 2016. 50 (4). P. 221–230.

McIntosh R.A., Dubcovsky J., Rogers W.J. Catalogue of gene symbols for wheat. SHIGEN. URL: (Last accessed: 21.03.2018).

Huerta-Espino J. Analysis of Wheat Leaf and Stem Rust Virulence on a Worldwide Basis: Ph.D. thesis. University of Minnesota, USA, 1992.

McFadden E.S. A successful transfer of emmer characters to vulgare wheat. J. Am. Soc. Agron. 1930. Vol. 22. P. 1020–1034.

Jin Y., Singh R.P., Ward R.W. Characterization of seedling infection types and adult plant infection responses of monogenic Sr gene lines to race TTKS of Puccinia graminis f. sp. tritici. Plant Disease. 2007. Vol. 91. P. 1096–1099.

Singh S., Singh R.P., Bhavani S., Huerta-Espino J., Eugenio L.V. QTL mapping of slow-rusting, adult plant resistance to race Ug99 of stem rust fungus in PBW343/Muu RIL population. Theor. Appl. Genet. 2013. Vol. 126(5). P. 1367–1375.

McIntosh R.A. Genetic and cytogenetic studies of stem rust, leaf rust and powdery midew resistances in Hope and related wheat cultivars. Austral. J. Biol. Sci. 1967. Vol. 20. P. 1181–1192.

Hare R.A. Genetic and cytogenetic studies of durable adult-plant resistance in Hope and related cultivars to wheat rusts. Z. Pflanzenzuecht. 1979. Vol. 83. P. 350–367.

Mago R., Brown-Guedira G., Dreisigacker S. An accurate DNA marker assay for stem rust resistance gene Sr2 in wheat. Theor. Appl. Genet. 2011. Vol. 122(4). P. 735–744.

Sunderwirth S.D. Greenhouse evaluation of the adult plant resistance of Sr2 to wheat stem rust. Phytopathology. 1980. Vol. 70. P. 634–637.

Kerber E.R. Resistance to leaf rust in hexaploid wheat: Lr32 a third gene derived from Triticum tauschii. Crop Sci. 1987. Vol. 27. P. 204–206.

Jones S.S., Dvorak J., Knott D.R., Qualset C.O. Use of double-ditelosomic and normal chromosome 1D recombinant substitution lines to map Sr33 on chromosome arm 1DS in wheat. Genome. 1991. Vol. 34. P. 505–508.