The ability of maize Lancaster inbreds to callusogenesis in vitro under varying environmental conditions

  • K. V. Derkach
  • V. V. Borysova
  • V. O. Maletskyi
  • T. M. Satarova


Aim. This work is focused on the estimation of the callusogenic potential of 10 maize Lancaster germplasm inbreds in comparison with well-known inbreds-standards with high callusogenic ability A188, Chi31 and PLS61, and the identification of genotypes with stable morphogenic callus formation under varying conditions of donor plant cultivation during three years of researches. Methods. Method of cell, tissue and organ culture in vitro. Field method. One-way and two-way analysis of variance. Results. For the investigated Lancaster inbreds the average multi-annual value of the total frequency of callusogenesis was 80.1 %, the frequency of morphogenic type I callus formation was 25.7 %, type II callus formation was 43.8 %, but for inbreds-standards these ones reached, respectively, 96.2 %, 12.2 % and 65.4 %. The level of callusogenesis varied depending on the year of investigations for all studied genotypes. The influence of genotype, year conditions and the combination of these factors on callus induction for most of the studied inbreds was significant. Conclusions. The impact of the interaction of a genotype and ecological factors of donor plant cultivation on morphogenic callusogenesis of type I as well as type II was the most significant forLancaster inbreds.Lancaster inbreds ДK298, ДK6080, ДK212 and ДK420-1 were the most stable under varying environmental conditions on the total frequency of callusogenesis, the frequencies of morphogenic and type II callus formation. None of the studied inbreds revealed the stability on type I callus formation frequency.

Keywords: maize (Zea mays L.), Lancaster germplasm, callusogenesis, culture in vitro.


Green C.E., Phillips H.L. Plant regeneration from tissues cultures of maize. Crop Science. 1975. Vol. 15, No. 5. P. 417–421.

Carvalho C., Bohorova N., Bordallo P., Abreu L., Valicente F., Bressan W., Paiva E. Type II callus production and plant regeneration in tropical maize genotypes. Plant Cell Reports. 1997. Vol. 17. P. 73–76. doi: 10.1007/s002990050355.

Frame B.R., Zhang H., Cocciolone S.M., Sidorenko L.V., Dietrich C.R., Pegg S.E., Zhen S., Schnable P.S., Wang K. Production of transgenic maize from bombarded type II callus: Effect of gold particle size and callus morphology on transformation efficiency. In Vitro Cell. Dev. Biol. Plant. 2000. Vol. 36. P. 21–29. doi: 10.1007/s11627-000-0007-5.

Raji J.A., Frame B., Little D., Santoso T.J., Wang K. Agrobacterium- and biolistic-mediated transformation of maize B104 inbred. Methods Mol. Biol. 2018. Vol. 1676. P. 15–40. doi: 10.1007/978-1-4939-7315-6_2.

Guruprasad M., Sridevi T.V., Udaya Sri A.P.P., Kumar M.S. Plant regeneration through callus initiation from mature and immature embryos of maize (Zea mays L.). Internat. J. Multidisciplinary. Advanced Research Trends. 2015. Vol. 2, No. 1. P. 195–202. doi: 10.18805/ijare.v0iOF.8435

Malini N., Ananadakumar C.R., Hariramakrishnan S. Regeneration of Indian maize genotypes (Zea mays L.) from immature embryo culture through callus induction. Journal of Applied and Natural Science. 2015. Vol. 7, No. 1. P. 131–137. doi: 10.31018/jans.v7i1.576

Kunah V.A. Biotechnology of medicinal plants. Genetic and physiological and biochemical bases. Kyiv: Logos, 2005. 730 p.

Nicoglou A. The evolution of phenotypic plasticity: genealogy of a debate in genetics. Stud. Hist. Philos. Biol. Biomed. Sci. 2015. Vol. 50. P. 67–76. doi: 10.1016/j.shpsc.2015.01.003.

Dzyubetsky B.V., Bodenko N.A., Fedko M.M., Gusak Yu.V. Creation of medium late maize hybrids based on Lancaster germplasm (C103). Biuleten′ Instytutu sil′s′koho hospodarstva stepovoï zony. 2012. No. 3. P. 8–11.

Abraimova O.E., Piralov G.R., Satarova T.M. Biotechnological characteristics of callusogenesis in maize immature embryo culture under the influence of abscisic acid and 6-benzylaminopurine. Visnyk of Dnipropetrovsk University. Biology. Medicine. 2010. Vol. 1, No. 1. P. 3–8.

Van Lammeren A.A.M. Observation on the structural development of immature maize embryos (Zea mays L.) during in vitro culture in the presence or absence of 2,4-D. Acta botanica neerlandica. 1988. No. 37 (1). P. 49–61.

Piralov G.R., Abraimova O.E. Culture of the tissue of some genotypes of maize of foreign selection. Faktory eksperymental′noï evoliutsiï orhanizmiv. 2008. Vol. 5. P. 309–313.

Nitovska I.O., Аbraimova О.Ye., Satarova T.M., Shakhovsky A.M., Morgun В.V. Biolistic transformation of immature maize embryos. Factors in experimental evolution of organisms. 2014. Vol. 15. P. 112–117.

Dzubetsky B.V., Bondar T.M. Combination ability of S3-S5 families, obtained on the basis of double sister hybrids of maize of genetic Iodent germplasm. Selektsiia i nasinnytstvo. 2014. Vol. 105. P. 16–22.

Chu C.C., Wang C.C., Sun C.S., Hsu C., Yin K.C., Chu C.Y. Establishment of an efficient medium for anther culture of rice through comparative experiments on nitrogen sources. Sci. Sinica. 1975. Vol. 18. P. 659–668.

Welham S.J., Gezan S.A., Clark S.J., Mead A. Statistical methods in biology: design and analysis of experiments and regression. CRC Press, Boca Raton, 2015. 608 p.