The combination of NO donor and ferulic acid effect on the elicitation of Triticum aestivum tolerance against Septoria tritici

  • I. V. Zhuk
  • A. P. Dmitriev
  • G. M. Lisova
  • L. O. Kucherova


Aim. The aim is to research the ability of enhancing ferulic acid effect as a biotic elicitor to induce tolerance of winter wheat plants against Septoria tritici Rob et Desm. leaf blotch infection by addition donor of NO signal molecule. Methods. Content of endogenous H2O2 was measured in wheat leaves (cv. Oberig myronivskij and Svytanok myronivskij). The extent of disease development, morphometric parameters and yield structure were analyzed. Results. It is shown that combination of treatment by ferulic acid with NO donor reduced the disease symptoms on 1–2 points. The level of endogenous hydrogen peroxide increased on 27 % in cv. Svytanok myronivskij. Conclusions. The data obtained suggest that combination of ferulic acid with donor NO could be used as more effective combination than biotic elicitor. They decreased the degree of lesions in leaf area caused by Septoria tritici Rob et Desm. leaf blotch infection and stimulated the growth of wheat plants. The analyze of biochemical mechanisms revealed that system resistance of wheat plants is induced via activation of antioxidant protection.

Keywords: ferulic acid, NO, biotic elicitors, induced resistance, Triticum aestivum L., Septoria tritici Rob et Desm.


Dmitriev A.P., Kovbasenko R.V., Avdeeva L.V., Lapa S.V., Kovbasenko V.M. Signal systems of plants and tolerance formation against biotic stress. Kyiv: Fenix, 2015. 192 p.

Tyuterev S.L. Ecologically safe elicitors of plant tolerance to diseases and physiological stresses. Plant Protection News. 2015. Vol. 1 (83). P. 3–13.

Zhuk I.V., Dmitriev A.P., Lysova G.M. The specifics of elicitor effect on Triticum aestivum L. macromorphogenesis under simultaneous lesion by Septoria tritici and Puccinia recondite. Modern Phytomorphology. 2016. Vol. 10. P. 117–123. doi: 10.5281/zenodo.155369

Zhuk I.V., Dmitriev A.P., Lysova G.M. Rol` peroksy`dazy` u formuvanni indukovanoyi stijkosti rosly`n psheny`ci za dopomogoyu imunomodulyatoriv. Factors in experimental evolution of organisms. 2016. Vol. 18. P. 81–84.

Zhuk I.V., Dmitriev A.P., Lysova G.M. The influence of oxalic acid and sodium nitroprusside on winter wheat productivity and tolerance to septoria leaf blotch and rust. Bulletin of Kharkiv National agrarian university. Biology. 2017. Vol. 2 (41). P. 68–76.

Zhuk I.V, Dmitriev A.P., Lysova G.M., Kucherova L.O. Participation of ferulic acid in elicitation of winter wheat plants resistance against Septoria tritici infection. Factors in experimental evolution of organisms. 2017. Vol. 20. P. 190–193.

Babayants O.V., Babayants L.T. Basis of selection and methodology of wheat tolerance estimation to diseases agents. Odessa, 2014. 401 p.

Nakano Y., Asada K. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol. 1981. Vol. 22. P. 867–880.

Rudd J.J., Kanyuka K., Hassani-Pak K., Derbyshire M., Andongabo A., Devonshire J., Lysenko A., Saqi M., Desai N.M., Powers S. J., Hooper J., Ambroso L., Bharti A., Farmer A., Hammond-Kosack K.E., Dietrich R.A., Courbot M. Transcriptome and metabolite profiling of the infection Cycle of Zymoseptoria tritici on wheat reveals a biphasic interaction with plant immunity involving differential pathogen chromosomal contributions and a variation on the hemibiotrophic lifestyle definition. Plant Physiology, March 2015. Vol. 167. P. 1158–1185. doi: 10.1104/pp.114.255927

Kao Y.-T., Gonzalez K.L., Bartel B. Peroxisome function, biogenesis, and dynamics in plants. Plant Physiology, January 2018. Vol. 176, P. 162–177. doi: 10.1104/pp.17.01050

Moural T.W., Lewis K.M., Barnaba C., Zhu F., Palmer N.A., Sarath G., Scully E.D., Jones J.P., Sattler S.E., Kang Ch.H. Characterization of class III peroxidases from switchgrass. Plant Physiol. Jan 2017. Vol. 173 (1). P. 417–433. doi: 10.1104/pp.16.01426

Schmidt R., Kunkowska A.B., Schippers J.H.M. Role of reactive oxygen species during cell expansion in leaves. Plant Physiol. Dec. 2016. 72 (4). P. 2098–2106. doi: 10.1104/pp.16.00426