The primary culture of malignant glioma cells as a model for the study of anti-tumor activity of substances

  • I. M. Shuba The State Institution «Romodanov Neurosurgery Institute, NAMS of Ukraine» Ukraine, 04050, Kyiv, Mayborody st., 32
  • V. V. Lylo Institute of Molecular Biology and Genetics of Natl. Acad. Sci. of Ukraine, Ukraine, 03143, Kyiv, Akad. Zabolotnogo str., 150
  • I. S. Karpova Institute of Molecular Biology and Genetics of Natl. Acad. Sci. of Ukraine, Ukraine, 03143, Kyiv, Akad. Zabolotnogo str., 150; Institute of Plant Physiology and Genetics NAS of Ukraine, Ukraine, 03022, Kyiv, Vasylkivska st., 31/17
  • O. Y. Glavatskyi The State Institution «Romodanov Neurosurgery Institute, NAMS of Ukraine» Ukraine, 04050, Kyiv, Mayborody st., 32
  • O. I. Kornelyuk Institute of Molecular Biology and Genetics of Natl. Acad. Sci. of Ukraine, Ukraine, 03143, Kyiv, Akad. Zabolotnogo str., 150


Aim. The aim of our work was to optimize the scheme of obtaining primary cell culture of malignant gliomas, which can be a model for a personalized approach in the selection of chemotherapeutic exposure tactics. Methods. The standard glioma cell line U-251MG and cells obtained as a result of mechanical disaggregation of Gr III–IV tumor fragments to single isolated cells were used. Results. A comparative analysis of the results of cultivation of the standard glioma cell line U-251MG and the primary cell culture of malignant gliomas. An optimized scheme for obtaining primary cultures of human malignant glioma cells isolated from glial tumor fragments obtained during surgery is proposed. Conclusions. Today, more and more preferred methods of individual determination of chemosensitivity over the appointment of standard chemotherapy regimens and it is the primary culture of tumor cells, from our point of view, can be used to test the response to the effect of chemotherapy.
Keywords: malignant glioma cells, primary culture, standard cell line.


International agency for research of cancer (Globocan) [Electronic resource]: The World of Health Organization. 2012. Retrived from

Stepanenko A. A., Kavsan V. M. Evolutionary karyotypic theory of cancer versus conventional cancer gene mutation theory. Biopolymers and Cell. 2012. Vol. 28, N. 4. P. 267–280. doi: 10.7124/bc.000059

Duesberg P., Fabarius A., Hehlmann R. Aneuploidy, the primary cause of the multilateral genomic instability of neoplastic and preneoplastic cells. IUBMB Life. 2004. N 2. P. 65–81. doi: 10.1080/15216540410001667902

Zeng T., Cui D., Gao L. Glioma: an overview of current classifications, characteristics, molecular biology and target therapies. Front Biosci. 2015. N 20. P. 1104– 1115. doi: 10.2741/4362.

Zozulia Yu. A., Vasileva I. G., Glavatskyi A. Ya. et al. Gliomas of the brain. (edited by a Yu. A. Zozulia). Kyiv: UYPK “EksOb”, 2007. 630 p.

Feniksov V. M. Clinical and molecular biological prognostic factors of brain gliomas: avtoref. dis.. kand. med. nauk. Moskva, 2010. 19 p.

Shoemaker R. H. The NCI60 human tumour cell line anticancer drug screen. Nat. Rev. Cancer. 2006. V. 6, N 10. P. 813–823. doi: 10.1038/nrc1951.

Svirnovski A. I. Leukemia therapy personalization: role of some laboratory technologies. Medical News. 2013; 9: 6–11.

Popov B. V., Petrov N. S., Mikhaylov V. M. Spontaneous transformation and immortalization of mesenchymal stem cells in an in vitro culture. Tsitologiia. 2009. V. 51(2). P. 91–102.

Freshney R. J. Culture of animal cells. A Manual of Basic Technique / fifth edition R. Jan Freshney. Bionom. 2010. P. 714.

Stepanenko A., Andreieva S., Korets K. Mykytenko D., Huleyuk N., Vasstzky Y., Kavsan V. Step-wise and punctuated genome evolution drive phenotype changes of tumor cells. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. 2015. Vol. 771, N 2. P. 56–69. doi: 10.1016/j.mrfmmm.2014.12/2016

Glavatskyi A. Ya., Volchenskova Y. Y., Gladkyy A. V., Semenova V. M. In vitro study of the cytotoxic activity of polyplatillen and its composition with fluorouracil. Zbirnyk naukovykh prats’ spivrobitnykiv NMAPO im. P. Shupika. 2006, vyp.15, kn. 2. P. 13–20.

Semenova V. M. Isolation and cultivation of nerve tissue cells. Cell Culture Methods / edited by a G. P. Pinaeva, M. S. Bogdanovoy. – SPb.: Izd-vo Politekhn. un-ta. Sankt-Peterburg. 2008. P. 157–176.

Galderisi F., Stork L., Li J., Mori M., MongoueTchokote S., Huang J. Flow cytometric chemosensitivity assay as a predictive tool of early clinical response in acute lymphoblastic leukemia. Pediatr. Blood Cancer. 2009. Vol. 53. P. 543-550. doi: 10.1002/pbc.22119.

Strickland S. A., Raptis A., Hallquist A. Correlation of the microculture-kinetic drug-induced apoptosis assay with patient outcomes in initial treatment of adult acute myelocytic leukemia. Leuk. Lymph. 2013. Vol. 54. P. 528-534. doi: 10.3109/10428194.2012.722217.

Glavatskyi A. Ya., Volchenskova Y. Y., Semenova V. M., Khmelnitskiy G. V., Shchalimov S. A., Maydanevich N. N., Stayno L. P. The effect of a platinum derivative with deoxyribonucleic acid on the survival of cultured brain tumor cells. Fizika zhivogo. 2001. V. 9, N 2, P. 7–13.

Strober W. Trypan blue exclusion test of cell viability. Curr Protoc Immunol. 2001 doi: 10.1002/0471142735.ima03bs21.

David N. L., Arie P., Guido R., Deimling A., FigarellaBranger D., Cavenee W. K., Ohgaki H., Wiestler O. D., Kleihues P., Ellison D. W. The 2016 World Health Organization classification of tumors of the central nervous system: a summary. Acta Neuropathol. doi: 10.1007/s00401-016-1545-1.

Lisianyy N. I., Orlov Iu. A., Kulik A. V., Lisianyy A. N., Potapova A. I. Diagnosis of virus-associated brain tumors. Ukr. Neurosurg. Journ. Kyiv. 2008, No. 1, P. 27– 31.

Semenova V. M., Stayno L. P. Methodological features of the cultivation of cells of the nervous tissue. Aspects of the application of tissue cultivation in neurobiology and neurooncology / V. M. Semenovа. Kiev: Interservis, 2018. P. 20–35.

Danilova R. K. Histology Guide Vol. 1, 2nd edition, revised and updated / Еd. by R. K. Danilova, Sankt-Peterburg: SpetsLit. 2010. P. 821.

U-251 MG Glioblastoma Cell Line. Retrieved October 09, 2019 from URL:

Khoruzhenko A. I. Two- and three-dimensional cell culture. Biopolymers and Cell. 2011. Vol. 27. N 1, P. 17–24. doi: 10.7124/bc.00007D

Li L., McCormack A. A., Nicholson J. M., Fabarius A., Hehlmann R., Sachs R. K. Cancer-causing karyotypes: chromosomal equilibria between destabilizing aneuploidy and stabilizing selection for oncogenic function. Cancer Genet Cytogenet. 2009. Vol. 188. P. 1–25. doi: 10.1016/j.cancergencyto.2008.08.016