Nucleotide sequences of tRNA-methonine genes of Streptomyces globisporus 1912-2, identified in silico

  • L. V. Polishchuk Institute of Microbiology and Virology of NAS of Ukraine, Ukraine, 03680, Kyiv, Akademika Zabolotnoho str., 154
DOI: https://doi.org/10.7124/visnyk.utgis.14.1.545

Abstract

Aim of this work was to identify nucleotide sequences of tRNAMet of S. globisporus 1912-2. Methods. Resources of server NCBI (programs BLAST: blast, discontiguous megablast and databases: “Genome”, “Nucleotide”) were used for in silico analysis of library of S. grlobisporus 1912-2 contigs. Results. Nucleotide sequences of 4 genes of tRNAMet of S. globisporus 1912-2 were determined in silico. Molecules of tRNA of the II type were translated from tRNAMet gene (Contig № 21 (936–1008 bp)) and the molecules of tRNAfMet genes (Contigs № 299 (1713–1787 bp), № 255 (5941–6015 bp)). Conclusions. 4 genes of transfer RNAs-methionine were identified in silico in S. globisporus 1912-2 genome. Two genes from them coded tRNAfMet molecules. The nucleotide sequences of all tRNAMet genes were identified.

Keywords: gene, tRNA, methionine, nucleotide sequence, Streptomyces.

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