Adhesion proteins are able to controle the proliferation and size of neonatal cardiomoycytes in Mus musculus

  • V. V. Balatskyi Institute of Molecular Biology and Genetics of Natl. Acad. Sci. of Ukraine, Ukraine, 03143, Kyiv, Akad. Zabolotnogo str., 150
  • L. L. Macewicz Institute of Molecular Biology and Genetics of Natl. Acad. Sci. of Ukraine, Ukraine, 03143, Kyiv, Akad. Zabolotnogo str., 150
  • T. P. Ruban Institute of Molecular Biology and Genetics of Natl. Acad. Sci. of Ukraine, Ukraine, 03143, Kyiv, Akad. Zabolotnogo str., 150
  • O. O. Piven Institute of Molecular Biology and Genetics of Natl. Acad. Sci. of Ukraine, Ukraine, 03143, Kyiv, Akad. Zabolotnogo str., 150

Abstract

Aim. In our present work, we have analyzed the influence of adhesion proteins — catenins on the proliferation of neonatal cardiomyocytes, under there cardiac-specific knockout. Methods. The studies were conducted using mice with a conditional knockout of the β-catenin gene (β-catflox/flox); αE-catenin (αE-catflox/flox) and transgenic animals which express the Crerecombinase under the control of the heavy chain promoter of α-myosin ((αMHC) -Cre). Results. The cardiac ablation of the β-catenin gene results in lower cell proliferation and decreased myocardial size, whereas the knockout of αE-catenin, increased proliferation as well as the size of the newborn heart. Conclusions. Intercellular adhesion genes — β-catenin and αE-catenins have not only an important structural function in maintaining of the myocardium tissue structure, but also involved in controlling of the proliferation, size of neonatal cardiomyocytes and newborn heart.
Keywords: β-catenin, αE-catenin, cardiomyocytes, proliferation.

References

Poss, K.D., Wilson, L.G, Keating, M.T. Heart regeneration in zebrafish. Science. 2002. Vol. 298. P. 2188–2190. doi: 10.1126/science.1077857

Oberpriller J.O, Oberpriller J.C. Response of the adult newt ventricle to injury. J. Exp. Zool. 1974. Vol. 187. P. 249–253. doi: 10.1002/jez.1401870208

Soonpaa M.H., Kim K.K., Pajak L., Franklin M., Field L.J. Cardiomyocyte DNA synthesis and binucleation during murine development. Am. J. Physiol. 1996. Vol. 271. P. H2183–H2189. doi: 10.1152/ajpheart.1996.271.5.H2183

Li F., Wang X., Capasso J. M., Gerdes, A. M. Rapid transition of cardiac myocytes from hyperplasia to hypertrophy during postnatal development. Journal of molecular and cellular cardiology. 1996. Vol. 28. P. 1737-1746. doi: 10.1006/jmcc.1996.0163

Porrello E. R., Mahmoud A. I., Simpson E., Hill J. A., Richardson J. A., Olson E. N., Sadek H. A. Transient regenerative potential of the neonatal mouse heart. Science. 2011. Vol. 331. P. 1078-1080. doi: 10.1126/science.1200708

Porrello E. R., Mahmoud A. I., Simpson E., Johnson B. A., Grinsfelder D., Canseco D., Mammen P. P., Rothermel B. A., Olson E. N., Sadek H. A. Regulation of neonatal and adult mammalian heart regeneration by the miR-15 family. Proceedings of the National Academy of Sciences of the United States of America. 2013. Vol. 110. P. 187-192. doi: 10.1126/science.1200708

Hirschy A., Schatzmann F., Ehler E., Perriard, J. C. Establishment of cardiac cytoarchitecture in the developing mouse heart. Developmental biology. 2006. Vol. 289. P. 430-441. doi: 10.1016/j.ydbio.2005.10.046

Takeichi M. Morphogenetic roles of classic cadherins. Curr Opin Cell Biol 1995. Vol. 7. P.619–627. doi: 10.1016/0955-0674(95)80102-2

Piven OO, Kostetskii IE, Macewicz LL, Kolomiets YM, Radice GL, Lukash LL. Requirement for N-cadherin-catenin complex in heart development. Exp Biol Med (Maywood). 2011. Vol. 236. P.816–22. doi: 10.1258/ebm.2011.010362

Balatskyi V. V., Akymenko I., Matsevych L. L., Piven O. O., Lukash L. L. Alfa-E-katenin u histolohichnykh perebudovakh miokarda pry starinni. Faktory eksperymentalnoi evoliutsii orhanizmiv. 2016. Vol. 18. P. 219-222.

Agah R., Frenkel P. A., French B. A. et al. Gene recombination in postmitotic cells targeted expression of cre recombinase provokes cardiac-restricted, site-specific rearrangement in adult ventricular muscle in vivo. The Journal of chemical physics. 1997. Vol. 100(1). P. 169–179. doi: 10.1172/JCI119509

Palchevska O. L., Balatskii V. V., Andrejeva A. O., Macewicz LL, Piven O. O., Lukash L.L. Embryonically induced β-catenin haploinsufficiency attenuates postnatal heart development and causes violation of foetal genes program. Biopolym. Cell. 2013. Vol. 29(2). P. 124–130. doi: 10.7124/bc.00080F

Lukash, L.L., Paton, E.B., Sukhorada E.M. Otsenky tsytotoksichnosti preparatov s antikantserogennym deistviem v kulturakh kletok cheloveka. Tsitologiia i genetika. 1997. Vol. 31(6). P. 26–34.

Twentyman P.R., Luscombe M. A study of some variables in a tetrazolium dye (MTT) based assay for cell growth and chemosensitivity. Br. J. Cancer. 1987. Vol. 56. P. 279–285.

Balatskyi V. V., Palchevska O. L., Matsevych L. L., Piven O. O. α-E-katenin potentsiinyi rehuliator kanonichnoho Wnt ta HIPPO-syhnalinhiv u miokardi. Visn. Ukr. tov. genet. selekts. 2016. Vol. 14(2). P. 168-173.