Peculiarities of the protein complex of seeds of pea (Pisum sativum L.)
Abstract
Aim. To investigate the features of the protein complex of pea seeds (Pisum sativum L.) of varieties of Ukrainian and foreign breeding of different morphotypes for use at the selection of varieties of food direction. Methods. Standard and developed in Laboratory of Plant Biochemistry methods of plant biochemical analysis (Kjeldahl method, spectrophotometric methods, electrophoresis). Statistical analysis of research results was carried out using the programs Libre Office Calc (GNU Lesser General Public Licensev3), “Imagel”. Results. The study of the content of protein, 11S and 7S globulins, their ratio in the seeds of peas of Ukrainian and foreign varieties showed the presence of reliable varietal differences according to the studied characteristics depending on their morphotype. Using electrophoretic and densitometric analyses, varietal differences in the intensity of bands, the presence or absence of some components in the electrophoretic spectra of 11S and 7S globulins, which affect the nutritional value of pea seeds, were established. Conclusions. The obtain results shown that it will be possible to select genotypes of food direction using the studied biochemical characteristics of evaluation.
References
Semba R. D., Ramsing R. , Rahman N. , Kraemer K. , Bloem M. W. Legumes as a sustainable source of protein in human diets. Global Food Security. 2021. Vol. 28. P. 100520. doi: 10.1016/j.gfs.2021.100520.
Dahl W. J., Foster L. M., Tyler R. T. Review of the health benefits of peas (Pisum sativum L.). Br. J. Nutr. 2012. Vol. 108 (l). P. 3–10. doi: 10.1017/S0007114512000852.
Carbonaro M., Nucara A. Legume proteins and peptides as compounds in nutraceuticals: a structural basis for dietary health effects. Nutrients. 2022. Vol. 14 (1188). P. 1–11. doi: 10.3390/nu14061188.
Tzitzikas E. N., Vincken J. P., de Groot J., Gruppen H., Visser R. G. Genetic variation in pea seed globulin composition. J. Agric. Food Chem. 2006. Vol. 54 (2). P. 425–433. doi: 10.1021/jf0519008.
Rubio L. A., Pérez A., Ruiz R., Guzmán M. A., Aranda‐Olmedo I., Clemente A. Characterization of pea (Pisum sativum) seed protein fractions. J. Sci. Food Agric. 2014. Vol. 94 (2). P. 280–287. doi: 10.1002/jsfa. 6250.
Koblay S. V. Adaptive capacity of different varieties of pea morfotype in conditions of the south of Ukraine. Breeding and seed production. 2016. Vol. 110. P. 82–90. [in Ukrainian]
Kjeldahl J. Neue Methode zur Bestimmung des Stickstoffs in organischen Körpern. Zeitschrift für analytische Chemie. 1983. Vol. 22 (1). P. 366–383. doi: 10.1007/BF01338151.
Adamovska V. G., Molodchenkova O. O., Kartuzova T. V., Sichkar V. I., Lavrova G. D. The method of selection of soybean genotypes of the food direction: Patent for utility model 107671 Ukraine; published on 24.06.2016. [in Ukrainian]
Adamovska V. G., Molodchenkova O. O., Sichkar V. I., Kartuzova T. V., Levitsky Yu. A., Bezkrovna L. Ya. Biochemical characteristics of genotypes of leguminous crops of the South of Ukraine in connection with seed quality breeding. Bulletin of Plant Breeding&Genetics Institute-National Center of Seed and Cultivar Investigation. 2015. Vol. 26 (66). P. 107–124. [in Ukrainian]
Taghvaei M., Sadeghi R., Smith R. Seed to seed variation of proteins of the yellow pea (Pisum sativum L.). PLoS ONE. 2022. Vol. 17 (8). e0271887. doi: 10.1371/journal.pone.0271887.
Sanchez Monge R., Lopez Torrejón G., Pascual C. Y., Varela J., Martin Esteban M., Salcedo G. Vicilin and convicilin are potential major allergens from pea. Clinical & Experimental Allergy. 2004. Vol. 34 (11). P. 1747–1753. doi: 10.1111/j.1365-2222.2004.02085.x.
