FNBP1 as a regulator of tumorigenesis: bioinformatic analysis and subcellular localization in chronic myeloid leukemia cells

DOI: https://doi.org/10.7124/FEEO.v39.1828
Keywords: FNBP1, TCGA, tumorigenesis, chronic myeloid leukemia, K562 cell line

Abstract

Aim. Formin-binding protein 1 (FNBP1) is an adaptor protein of the F-BAR family that plays a crucial role in the regulation of the actin cytoskeleton and membrane dynamics. Methods. Bioinformatic analysis of FNBP1 expression across multiple cancer types was performed using data from The Cancer Genome Atlas (TCGA) and the Genotype-Tissue Expression (GTEx) project. Immunofluorescence analysis of FNBP1 localization in K562 cells was conducted followed by confocal microscopy. Results. Bioinformatic analysis based on TCGA and GTEx datasets indicates the potential clinical relevance of FNBP1 as a biomarker and therapeutic target, as its expression correlates with patient prognosis and the level of immune infiltration. FNBP1 was predominantly localized in the cytoplasm, with partial accumulation in the perinuclear region, suggesting its involvement in vesicular trafficking, membrane remodeling, and cytoskeletal organization. Conclusions. The obtained data are consistent with the multifunctional role of FNBP1 in cytoskeletal dynamics and membrane trafficking, and further highlight its importance in cell migration and invasion, supporting its potential as a promising therapeutic target in malignancies.

References

Antonenko S., Gurianov D., Telegeev G. Colocalization of USP1 and PH domain of Bcr-Abl oncoprotein in terms of chronic myeloid leukemia cell rearrangements, Cytology and Genetics. 2016. Vol. 50. Р. 11–15. https://doi.org/10.3103/S0095452716050029.

Antonenko S., Kravchuk I., Telegeev G. Interaction of Bcr-Abl oncoprotein with the GlG1 protein in K562 cells: Its role in the pathogenesis of chronic myeloid leukemia. Cytology and Genetics. 2020. Vol. 54. Р. 48–54. https://doi.org/10.3103/s0095452720010028.

Antonenko S., Telegeev G. Inhibition of USP1, a new partner of Bcr-Abl, results in decrease of Bcr-Abl level in K562 cells. Experimental Oncology. 2020. Vol. 42. Р. 109–114. https://doi.org/10.32471/exp-oncology.2312-8852.vol-42-no-2.14533.

Antonenko S. V., Gurianov D. S., Kravchuk I. V. Role of BCR and FNBP1 proteins in phagocytosis as a model of membrane rearrangements with chronic myelogenous leukemia. Cytology and Genetics. 2023. Vol. 57. Р. 291–297. https://doi.org/10.3103/S0095452723040023.

Сancer Genetics. FNBP1. Retrieved from: https://www.cancer-genetics.org/FNBP1.htm.

FNBP1 – Formin-binding protein 1 – Homo sapiens (Human). UniProt. Retrieved from: https://www.uniprot.org/uniprotkb/H0Y7W6/entry.

GEPIA2. Retrieved from: http://gepia2.cancer-pku.cn/#analysis.

Katoh M., Katoh M. Identification and characterization of human FNBP1L gene in silico. International Journal of Molecular Medicine. 2004. Vol. 13 (1). Р. 157–162.

National Library of Medicine Gene: FNBP1. Retrieved from: https://www.ncbi.nlm.nih.gov/gtr/genes/23048/.

Taylor K. L., Taylor R. J., Richters K. E. Opposing functions of F-BAR proteins in neuronal membrane protrusion, tubule formation, and neurite outgrowth. Life Science Alliance. 2019. Vol. 2 (3). https://doi.org/10.26508/lsa.201800288.

Wang Z., Tian Z., Song X. Membrane tension sensing molecule-FNBP1 is a prognostic biomarker related to immune infiltration in BRCA, LUAD and STAD. BMC Immunology. 2022. Vol. 23 (1). https://doi.org/10.1186/s12865-021-00475-z.

Yoon B. K., Hwang N., Chun K. H. Sp1-Induced FNBP1 Drives Rigorous 3D Cell Motility in EMT-Type Gastric Cancer Cells. International Journal of Molecular Sciences. 2021. Vol. 22 (13). https://doi.org/10.3390/ijms22136784.

Zeni C., Komiya Y., Habas R. Formin Binding Protein 1 (FNBP1) regulates non-canonical Wnt signaling and vertebrate gastrulation. Developmental Biology. 2024. Р. 18–29. https://doi.org/10.1016/j.ydbio.2024.06.019.

Zhang J., Li X., Zhou Y., Lin M., Zhang Q., Wang Y. FNBP1 Facilitates Cervical Cancer Cell Survival by the Constitutive Activation of FAK/PI3K/AKT/mTOR Signaling. Cells. 2023. Vol. 12 (15). https://doi.org/10.3390/cells12151964.

Zhang L., Li F., Zhang L. RBM15B/IGF2BP2-m6A Mediated Upregulation of FNBP1 Promotes the Progression of Glioblastoma by Promoting Smad3-Mediated Glycolysis. Drug Development Research. 2025. Vol. 86 (7). https://doi.org/10.1002/ddr.70175.