Creation of new forms of feeding cereal grasses from A to Z

  • I. P. Kondratskaya
  • V. A. Stolepchenko
  • A. N. Yuknimuk
  • P. P. Vasko
  • O. V. Chizhik
  • Z. H. Kozlovskaya
  • A. M. Deeva
  • Т. V. Mazur
  • V. N. Reshetnikov
  • E. A. Voyzehovska
  • H. A. Pryadkina
  • S. Vasiljevic

Abstract

Aim. To create a new cultivars of perennial cereal grasses with the high feeding and seed productivity, longevity, drought resistance and freezing tolerance in order to increase the quality of livestock super-elite seeds production. DNA certification of economically valuable properties estimation. Methods. Hybridization, a partially known combination. Embryo culture method from immature caryopsis with completion of growing in a nutrient medium. Techniques of plant tissue culture (initiation, multiplication, rooting and adaptation in vitro plants to ex vitro conditions. DNA certification was performed using SSR (Simple Sequence Repeat) marker system. Results. The new competitively cultivar of Agropyron cristatum "Malarytsky" have been created. The system of the cultivar and parental forms registration in the form of molecular genetic passports is presented. Conclusions. The cultivar of Agropyron cristatum "Malarytsky" was turned over to the State Institution “The State Inspection for Testing and Protection of Plant Varieties” for state testing and for inclusion in the State Register of Varieties. The economic efficiency of this new feeding culture cultivating for Belarus and countries with sharply continental climate is presented.

Keywords: Agropyron cristatum, hybridization, cul populations, in vitro, water-soluble carbohydrates, DNA certification, molecular genetic passports, cultivar.

References

Soteriades A.D., Gonzalez-Mejia A.M., Styles D., Foskolos A., Moorby J.M., Gibbons J.M. Effect of high-sugar grasses and improved manure management of the environmental footprint of milk production at the farm level. Journal of Cleaner Production. 2018. Vol. 202. P. 1241–1252. doi: 10.1016/j.jclepro.2018.08.206.

Syvash O.O., Mykhailenko N.F., Zolotarova, O.K. Tsukry yak kliuchova lanka v rehuliatsii metabolizmu fotosyntezuiuchykh klityn. Ukr. Bot. Zhurnal. 2001. Vol. 58, No 1. P. 121–125. [in Ukrainian]

Bhowmik P.K., Tamura K., Sanada Y., Tase K., Yamada T. Sucrose Metabolism of Perennial Ryegrass in Relation to Cold Acclimation. Z. Naturforsch. C. 2006. Vol. 61, No. 1–2. Р. 99–104. doi: 10.1515/znc-2006-1-218.

Trouvelot S., Héloir M.C., Poinssot B., Gauthier A., Paris F., Guillier C., Combier M., Trdá L., Daire X., Adrian M. Carbohydrates in plant immunity and plant protection: roles and potential application as foliar sprays. Front Plant Sci. 2014. Vol. 5. P. 592. doi: 10.3389/fpls.2014.00592.

Sukhareva F.S., Kuluev B.R. DNA marker for genetic analysis of crops. Biomics. 2018. Vol. 10 (1). P. 69-84. doi:10.31301/2221-6197.bmcs.2018-15.

Guidelines for the selection of perennial grasses. Moskva, 1985. 188 p. [in Russian]

Ermakov A.I. Methods of biochemical research of plants. Agropromizdat, 1987. P. 122–142. [in Russian]

Yonghe Che, Yanping Yang, Xinming Yang, Xiuquan Li, Lihui Li Phylogenetic relationship and diversity among Agropyron Gaertn. germplasm using SSRs markers. Plant Syst Evol. doi: 10.1007/s00606-014-1062-4.

Humphreys M.O. Water-soluble carbohydratesin perennial ryegrass breeding. I. Genetic differencesamong cultivars and hybrid progeny grown as spacedplants. Grass and Forage Science. 1989. Vol. 44. P. 231–236.

Downing T., Gamroth R. Nonstructural carbohydrates in cool-season Grasses. 2007. Accessed from: http://extension.oregonstate.edu/catalog.pdf/sr/sr-1079-e.pdf.

Slewinski T.L. Non-structural carbohydrate partitioning in grass stems: a target to increase yield stability, stress tolerance, and biofuel production. J. Exp. Bot. 2012. Vol. 63, No. 13. Р. 4647–4670. doi: 10.1093/jxb/ers124.

Yang J., Zhang J., Wang Z., Zhu Q. Activities of starch hydrolytic enzymes and sucrose-phosphate synthase in the stems of rice subjected to water stress during grain filling. J. Exp. Bot. 2001. Vol. 52, No. 364. Р. 2169–2179.