The effect of UV-C explosion and exogenous cytokinin on pea plants

  • V. V. Zhuk Institute of Cell Biology and Genetic Engineering, National Academy of Science of Ukraine, Ukraine, 03143, Kyiv, Academika Zabolotnoho str., 148 https://orcid.org/0000-0003-1966-7537
  • O. M. Mikheev Institute of Cell Biology and Genetic Engineering, National Academy of Science of Ukraine, Ukraine, 03143, Kyiv, Academika Zabolotnoho str., 148
  • L. G. Ovsyannikova Institute of Cell Biology and Genetic Engineering, National Academy of Science of Ukraine, Ukraine, 03143, Kyiv, Academika Zabolotnoho str., 148
DOI: https://doi.org/10.7124/FEEO.v37.1759
Keywords: UV-C, Pisum sativum L., BAP, growth, HP, photosynthetic pigments

Abstract

Aim. The effect of ultraviolet C (UV-C) explosion and cytokinin benzylaminopurine (BAP) treatment on the growth shoots and roots, content of hydrogen peroxide (HP) and photosynthetic pigments in leaves of pea plants (Pisum sativum L.) was studied. Methods. Pea plants cultivar Aronis were irradiated by UV-C at a dose of 5 kJ/m2 with a power of 7 W/m2. Part of the non-irradiated plants were treated with BAP, part of the plants were treated with BAP one day after UV-C explosion. Plant shoots and roots length and mass were measured, content of photosynthetic pigments and HP in leaves were established during the experiment. Results. It was shown that UV-C explosion inhibited growth of pea plants, increased HP and decreased chlorophylls and carotenoid content in leaves. Treatment of plants with BAP after the UV-C explosion stimulated the accumulation of chlorophyll, but had no effect on HP and carotenoid content. Conclusions. It was shown that UV-C explosion of pea plants by dose of 5 kJ/m2 caused inhibition of growth, reduction photosynthetic pigments content, increased HP content in leaves. The BAP treatment after explosion stimulated the accumulation of chlorophylls content in leaves.

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