Вплив світлових умов і регуляторів росту на морфофізіологічні параметри рослин in vitro видів роду Carlina L.

Kh. M. Kolisnyk; L. R. Hrytsak; M. Z. Prokopiak; D. A. Boiko

doi:10.7124/FEEO.v37.1762

Kh. M. Kolisnyk V. Hnatiuk Ternopil National Pedagogical University, Ukraine, 46027, Ternopil, M. Kryvonosa str., 2
L. R. Hrytsak V. Hnatiuk Ternopil National Pedagogical University, Ukraine, 46027, Ternopil, M. Kryvonosa str., 2 https://orcid.org/0000-0002-2872-5201
M. Z. Prokopiak V. Hnatiuk Ternopil National Pedagogical University, Ukraine, 46027, Ternopil, M. Kryvonosa str., 2 https://orcid.org/0000-0002-2846-4208
D. A. Boiko V. Hnatiuk Ternopil National Pedagogical University, Ukraine, 46027, Ternopil, M. Kryvonosa str., 2 https://orcid.org/0000-0002-8927-8687

DOI: https://doi.org/10.7124/FEEO.v37.1762

PDF (Українська)

Keywords: Carlina L., plants in vitro, growth processes, composition of the nutrient medium, growth regulators, light intensity, photosynthetic apparatus

Abstract

Aim. To investigate the effects of growth regulators on the development of in vitro plants of Carlina species (Carlina onopordifolia Besser ex Szafer, Kulcz. et Pawł, Carlina cirsioides Klokov, and Carlina acaulis L.) and functioning of their photosynthetic apparatus under different light conditions. Methods. Spectrophotometric methods, in vitro plant cultivation techniques, statistical methods, biometric measurements were used. Results. It was established that the use of MS/2 medium for plant cultivation proved optimal for leaf formation in C. onopordifolia and C. cirsioides. However, this medium was completely unsuitable for root system development, resulting in the rapid death of micropropagated plants. The chemical composition of the nutrient medium was adjusted for C. onopordifolia, C. acaulis, and C. cirsioides in accordance with the elemental composition of soils from their natural habitats. The effectiveness of the addition of the growth regulator IAA and the commercial product Trevitan^® was shown to be species-specific. The optimal light intensity for each studied species under in vitro conditions was also determined. Conclusions. The results indicate that optimizing in vitro cultivation conditions for Carlina species requires consideration of the evolutionarily determined biological characteristics of each species.

References

Dias D. A., Urban S., Roessner U. A historical overview of natural products in drug discovery. Metabolites. 2012. No. 2. P. 303–336. https://doi.org/10.3390/metabo2020303

Yue W., Ming Q.L., Lin B. et al. Medicinal plant cell suspension cultures: Pharmaceutical applications and high-yielding strategies for the desired secondary metabolites. Critical Rev Biotechnol. 2016. No. 36 (2). P. 1–18. https://doi.org/10.3109/07388551.2014.923986

Isah T. Adjustments to in vitro culture conditions and associated anomalies in plants. Acta biologica cracoviensia. Ser. Botanica. 2015. No. 57/2. Р. 9–28.

Didukh Ya. P. Chervona knyha Ukrainy. Roslynnyi svit / Za red. Ya. P. Didukha. Kyiv : Hlobalkonsaltynh, 2009. 900 p. [in Ukrainian]

Andriienko T. L., Perehrym M. M. Ofitsiini pereliky rehionalno ridkisnykh roslyn administratyvnykh terytorii Ukrainy (dovidkove vydannia), Kyiv : Alterpres, 2012. 148 p. [in Ukrainian]

Melis A. Dynamics of photosynthetic membrane composition and function. Biochim. Biophys. Acta. 1991. Vol. 1058(1). P. 87–106. https://doi.org/10.1016/S0005-2728(05)80225-7

Murchie E. H. Acclimation of photosynthesis to irradiance and spectral quality in British plant species: chlorophyll content, photosynthetic capacity and habitat preference. Plant Cell Environ. 1997. Vol. 20, No. 2. P. 438–448. https://doi.org/10.1046/j.1365-3040.1997.d01-95.x

Tang W., Guo H., Baskin C. C. et al. Effect of Light Intensity on Morphology, Photosynthesis and Carbon Metabolism of Alfalfa (Medicago Sativa) Seedlings. Plants. 2022. Vol. 11. P. 1688. https://doi.org/10.3390/plants11131688

Qi J., Mao Y., Cui J. et al. The Role of Strigolactones in Resistance to Environmental Stress in Plants. Physiol. Plant. 2024. Vol. 176. e1441. https://doi.org/10.1111/ppl.14419

Dzendzel A. Yu., Pyda S. V. Rekultyvant kompozytsiinyi Trevitan™ – novyi kompleksnyi preparat dlia shvydkoi reheneratsii gruntu. Osvitni ta naukovi vymiry pryrodnychykh nauk : zbirnyk materialiv II Vseukrainskoi zaochnoi naukovoi konferentsii (m. Sumy, 8 hrudnia 2021 r.). Sumy : SumDPU im. A. S. Makarenka, 2021. P. 51–53. [in Ukrainian]

Lavnyi V. V. Fizyko-khimichni vlastyvosti gruntiv na deiakykh vitrovalnykh diliankakh Ukrainskykh Karpat. Naukovyi visnyk NLTU Ukrainy. 2012. Vyp. 22, 11. P. 66–70. [in Ukrainian]

Kravets N. B., Hrytsak L. R., Prokopiak M. Z., Maiorova O. Iu., Drobyk N. M. Vmist fotosyntetychnykh pihmentiv u roslynakh rodu Sarlina L. u pryrodi ta kulturi in vitro. Naukovi zapysky Ternopilskoho natsionalnoho pedahohichnoho universytetu imeni Volodymyra Hnatiuka. Seriia: Biolohiia. 2019. No. 4 (78). P. 16–23. [in Ukrainian]

Liuta I. Vplyv skladu zhyvylnykh seredovyshch na rist i rozvytok eksplantiv vynohradu, otrymanykh v umovakh in vitro. International Science Journal of Engineering & Agriculture. 2024. 3(6):107-116 (in Ukrainian). http://doi.org/10.46299/j.isjea.20240306.11.

Gao J., Zhuang S., Zhang W. Advances in Plant Auxin Biology: Synthesis, Metabolism, Signaling, Interaction with Other Hormones, and Roles under Abiotic Stress. Plants. 2021. Vol. 13. P. 2523. https://doi.org/10.3390/plants13172523.

Syvash O. O. Akumuliatsiia soniachnoi enerhii: fotosyntez chy shtuchni systemy. Biotechnologia Acta. 2012. Vol. 5(6). P. 27–38. [in Ukrainian]

Influence of ligh conditions and growth regulators on morpho-physiological characteristics of in vitro plants of Carlina L. species

Abstract

References

Contacts