Design of DNA markers for intron length polymorphism analysis of α- and γ-tubulin genes in macromycetes

  • D. O. Novozhylov Institute of Food Biotechnology and Genomics, the National Academy of Sciences of Ukraine, Ukraine, 04123, Kyiv, Baidy-Vyshnevetskoho str., 2a https://orcid.org/0009-0008-1491-7166
  • T. S. Shut Institute of Food Biotechnology and Genomics, the National Academy of Sciences of Ukraine, Ukraine, 04123, Kyiv, Baidy-Vyshnevetskoho str., 2a https://orcid.org/0009-0008-3849-599X
  • A. M. Rabokon Institute of Food Biotechnology and Genomics, the National Academy of Sciences of Ukraine, Ukraine, 04123, Kyiv, Baidy-Vyshnevetskoho str., 2a https://orcid.org/0000-0002-6249-1824
  • R. Y. Blume Institute of Food Biotechnology and Genomics, the National Academy of Sciences of Ukraine, Ukraine, 04123, Kyiv, Baidy-Vyshnevetskoho str., 2a https://orcid.org/0000-0003-4936-1803
  • A. Y. Demkovych Institute of Food Biotechnology and Genomics, the National Academy of Sciences of Ukraine, Ukraine, 04123, Kyiv, Baidy-Vyshnevetskoho str., 2a https://orcid.org/0000-0002-7433-1203
  • S. M. Pryvalikhin Institute of Food Biotechnology and Genomics, the National Academy of Sciences of Ukraine, Ukraine, 04123, Kyiv, Baidy-Vyshnevetskoho str., 2a https://orcid.org/0009-0006-2462-2105
  • D. M. Mykhailova Educational and Scientific Center "Institute of Biology and Medicine", Taras Shevchenko National University of Kyiv, Ukraine, 03022, Kyiv, Academika Hlushkova ave., 2 https://orcid.org/0009-0000-3669-9552
  • Ya. V. Pirko Institute of Food Biotechnology and Genomics, the National Academy of Sciences of Ukraine, Ukraine, 04123, Kyiv, Baidy-Vyshnevetskoho str., 2a https://orcid.org/0000-0003-1887-5406
  • Ya. B. Blume Institute of Food Biotechnology and Genomics, the National Academy of Sciences of Ukraine, Ukraine, 04123, Kyiv, Baidy-Vyshnevetskoho str., 2a https://orcid.org/0000-0001-7078-7548
DOI: https://doi.org/10.7124/FEEO.v38.1801
Keywords: genotyping, fungi, exon-intron structure, α-tubulin, γ-tubulin

Abstract

Aim. Development of markers for determining Intron Length Polymorphism (ILP) of α- and γ-tubulin genes of macromycetes. Methods. Use of bioinformatic methods for search of gene loci encoding α- and γ-tubulins in macromycetes, analysis of exon-intron structure of genes, cladistic analysis, design of ILP markers, and verification of the designed primers using in silico PCR. Results. The exon-intron structure of α- and γ-tubulin genes in macromycetes was analyzed. Potential markers were designed to analyze the polymorphism of introns 1–3 of α-tubulins in Basidiomycetes, introns 2–4 of α-tubulins in Ascomycetes, and a set of six of potential markers for analyzing the polymorphism of introns 1, 2, 3, and 5 of γ-tubulins in Basidiomycetes. Conclusions. The feasibility of creating highly universal ILP markers based on α- and γ-tubulin gene sequences in macromycetes has been demonstrated. A series of potential ILP markers suitable for fungal genotyping has been proposed.

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