Phylogenetic characterisation and antibiotic activity of natural isolates of the genus Saccharopolyspora

  • S. I. Tistechok Ivan Franko National University of Lviv, Ukraine, 79005, Lviv, Universytetska str., 1; Explogen LLC, Ukraine, 79053, Lviv, Volodymyra Velykogo str., 16 https://orcid.org/0000-0003-2116-746X
  • S. B. Markevych Ivan Franko National University of Lviv, Ukraine, 79005, Lviv, Hrushevsky str., 4
  • S. A. Pinchuk Ivan Franko National University of Lviv, Ukraine, 79005, Lviv, Hrushevsky str., 4
  • V. O. Fedorenko Ivan Franko National University of Lviv, Ukraine, 79005, Lviv, Hrushevsky str., 4 https://orcid.org/0000-0002-7672-1897
  • O. M. Gromyko Ivan Franko National University of Lviv, Ukraine, 79005, Lviv, Hrushevsky str., 4 https://orcid.org/0000-0002-8107-0128
DOI: https://doi.org/10.7124/FEEO.v38.1805
Keywords: actinomycetes, Saccharopolyspora, phylogenetic analysis, antimicrobial activity

Abstract

Aim. Rare genera of actinomycetes, such as the genus Saccharopolyspora, are a valuable source of bioactive compounds with diverse activities. The aim of this study was to investigate the phylogeny of natural isolates of rhizospheric actinomycetes from the Crimean Peninsula belonging to the genus Saccharopolyspora and their potential to produce antibiotic compounds. Methods. Microbiological, bioinformatic, molecular genetics, and statistical methods were employed in this study. Results. The phylogeny of the rhizospheric actinomycetes within the genus Saccharopolyspora was investigated using nucleotide sequences of the 16S rRNA, gyrB, and rpoB genes. Intrageneric differentiation of isolates within the genus Saccharopolyspora was demonstrated. It was established that the isolates Saccharopolyspora sp. Oe 2-284 and Saccharopolyspora sp. Oa 2-139 likely belong to the same species. It was found that the antimicrobial activity of the isolates depends significantly on the cultivation conditions. The most pronounced antibiotic activity was demonstrated by the Saccharopolyspora sp. Oe 2-284 isolate, which inhibited the growth of both Gram-positive and Gram-negative bacteria. The isolate Oa 2-139 exhibited moderate activity, whereas isolates Je 1-415 and Ya 6-223 showed no antimicrobial activity against the test microorganisms used. None of the isolates exhibited activity against Candida albicans Berkhout. Conclusions. Phylogenetic analysis based on the 16S rRNA, gyrB, and rpoB genes affiliated the studied isolates with the genus Saccharopolyspora. Certain isolates of the genus Saccharopolyspora demonstrate biosynthetic potential to produce biologically active compounds with antimicrobial activity.

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